Interaction of Plum Pox Virus with Specific Colloidal Gold-Labeled Antibodies and Development of Immunochromatographic Assay of the Virus
| Autor: | Anatoly V. Zherdev, Irina V. Safenkova, V. G. Avdienko, S. N. Chirkov, Irina Mitrofanova, A. N. Guseva, Boris B. Dzantiev, Nadezhda A. Byzova, Joseph Atabekov |
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| Rok vydání: | 2010 |
| Předmět: |
medicine.drug_class
Enzyme-Linked Immunosorbent Assay Gold Colloid Biology Monoclonal antibody Biochemistry Colorimetry (chemical method) Virus Mice Limit of Detection Virology Plant virus medicine Animals Particle Size Plant Diseases Reagent Strips Detection limit Mice Inbred BALB C Chromatography Antibodies Monoclonal Flocculation General Medicine Immunohistochemistry Plant Leaves Plum Pox Virus Colloidal gold biology.protein Nanoparticles Colorimetry Chromatography Thin Layer Prunus Antibody Conjugate |
| Zdroj: | Biochemistry (Moscow). 75:1393-1403 |
| ISSN: | 1608-3040 0006-2979 |
| Popis: | Two monoclonal antibodies (mABs) raised against plum pox virus (PPV) were shown to recognize its D, M, and C strains. Conjugates of the antibodies with colloidal gold (CG) nanoparticles averaging 26 nm in diameter were synthesized. The binding constants of PPV with both the native and conjugated mABs were determined using a Biacore X device. The complexes between the CG-mAB conjugates and plum pox virions were examined by means of transmission electron and atomic force microscopy. Using the conjugates with optimal component ratio, an express immunochromatographic assay of PPV was developed with a detection limit of 3 ng/ml and duration of 10 min. The assay was tested for PPV detection in sam- ples of stone fruit tree leaves and demonstrated a good compatibility with the data obtained by “sandwich”-ELISA. The developed assay can be used in the field and applied for monitoring viral infection and for quarantine purposes. |
| Databáze: | OpenAIRE |
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