Activity of antioxidant defense enzymes and glutathione content in some tissues of the Belgrade (b/b) laboratory rat
| Autor: | Bato Korac, V.M. Petrović, Duško Blagojević, Zorica S. Saičić, M. Simović, Mihajlo B. Spasić, B. Buzadžić |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Immunology Glutathione reductase Spleen Antioxidants chemistry.chemical_compound Internal medicine Testis medicine Animals Rats Inbred BB Lung Pharmacology chemistry.chemical_classification biology Glutathione peroxidase Myocardium Brain Glutathione Rats medicine.anatomical_structure Endocrinology Glutathione S-transferase chemistry Liver Catalase biology.protein Dismutase Female Oxidoreductases |
| Zdroj: | Comparative Biochemistry and Physiology C-Pharmacology Toxicology & Endocrinology |
| ISSN: | 1367-8280 |
| Popis: | The activity of antioxidant defense (AD) enzymes-superoxide dismutase (SOD, EC 1.15.1.1.), catalase (CAT, EC 1.11.1.6.), glutathione peroxidase (GSH-Px, EC 1.11.1.9.), glutathione-S-transferase (GST, EC 2.5.1.18), glutathione reductase (GR, EC 1.6.4.2) and glutathione (GSH) content of the anemic Belgrade (b/b) laboratory rats-were measured and analyzed in liver, spleen, lung, heart, brain and testes in comparison with nonanemic controls. The activities of hepatic Mn SOD, CAT, GSH-Px and GST (P < 0.02, P < 0.01 and P < 0.005) were decreased in anemic, comparing with nonanemic animals, whereas the spleen CuZn SOD, Mn SOD, CAT and GSH-Px (P < 0.005, P < 0.02, P < 0.005 and P < 0.01) activities were increased. In the lung of anemic rats, Mn SOD, GSH-Px and GR (P < 0.005, P < 0.01, P < 0.05) activities were higher, whereas GST (P < 0.01) activity was lower in relation to nonanemic ones. In anemic rats, heart Mn SOD (P < 0.05) activity was increased, brain GSH-Px (P < 0.005) activity was lower, whereas GR (P < 0.02) activity was higher compared with nonanemic controls. CuZn SOD (P < 0.05) activity in the testes was elevated and GSH-Px (P < 0.05) reduced in anemic animals. GSH content was decreased in the liver (P < 0.01), lung and brain (P < 0.005) and increased in the spleen (P < 0.02) of anemic rats in relation to the controls. Our data suggest phenotype specific differences in the AD system of the Belgrade (b/b) rat tissues in comparison with nonanemic controls. (C) 1997 Elsevier Science Inc. null |
| Databáze: | OpenAIRE |
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