Inflammation-Induced Acute Phase Response in Skeletal Muscle and Critical Illness Myopathy
| Autor: | Jida Hamati, Michael Boschmann, Herbert Schulz, Jens Fielitz, Xiaoxi Zhu, Claudia Spies, Claudia Langhans, Joachim Spranger, Vincent Mouly, Siegfried Labeit, Tobias Wollersheim, Steffen Weber-Carstens, Gunnar Dittmar, Norbert Hubner, Kathrin Saar, Franziska Schmidt, Melanie Kny, Susanne Koch, Martin Krebs, Simone Spuler, Doerte Lodka, Gillian Butler-Browne |
|---|---|
| Přispěvatelé: | Max Delbrück Center, Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Universität Mannheim [Mannheim], Institut de Myologie, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Association française contre les myopathies (AFM-Téléthon)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Pierre et Marie Curie - Paris 6 (UPMC), HAL UPMC, Gestionnaire, Université Pierre et Marie Curie - Paris 6 (UPMC)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Association française contre les myopathies (AFM-Téléthon)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universität Mannheim |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Lipopolysaccharides
Male Pathology Critical Illness Myopathy Muscle Physiology Critical Care and Emergency Medicine Muscle Functions Physiology Systemic inflammation Pathology and Laboratory Medicine Gastroenterology Muscular Dystrophies Mice 0302 clinical medicine Medicine and Health Sciences Myocyte Musculoskeletal System Immune Response Oligonucleotide Array Sequence Analysis 0303 health sciences Multidisciplinary [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Muscles Neuromuscular Diseases Middle Aged 3. Good health medicine.anatomical_structure Neurology Research Design Observational Studies Medicine Multiple Organ Dysfunction Syndrome Female medicine.symptom Technology Platforms Anatomy Inflammation Mediators Function and Dysfunction of the Nervous System Research Article Adult medicine.medical_specialty Clinical Research Design Science Critical Illness Immunology Inflammation Research and Analysis Methods Microbiology Sepsis 03 medical and health sciences Signs and Symptoms Muscular Diseases Intensive care Internal medicine medicine Animals Humans Animal Models of Disease Myopathy Acute-Phase Reaction Muscle Skeletal 030304 developmental biology Serum Amyloid A Protein Membranes business.industry Interleukin-6 Tumor Necrosis Factor-alpha Immunity Skeletal muscle Biology and Life Sciences medicine.disease Gene Expression Regulation Cardiovascular and Metabolic Diseases Case-Control Studies Clinical Immunology business 030217 neurology & neurosurgery [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (3), pp.e92048. ⟨10.1371/journal.pone.0092048⟩ PLoS ONE, 2014, 9 (3), pp.e92048. ⟨10.1371/journal.pone.0092048⟩ PLoS ONE, Vol 9, Iss 3, p e92048 (2014) |
| ISSN: | 1932-6203 |
| Popis: | ObjectivesSystemic inflammation is a major risk factor for critical-illness myopathy (CIM) but its pathogenic role in muscle is uncertain. We observed that interleukin 6 (IL-6) and serum amyloid A1 (SAA1) expression was upregulated in muscle of critically ill patients. To test the relevance of these responses we assessed inflammation and acute-phase response at early and late time points in muscle of patients at risk for CIM.DesignProspective observational clinical study and prospective animal trial.SettingTwo intensive care units (ICU) and research laboratory.Patients/subjects33 patients with Sequential Organ Failure Assessment scores ≥ 8 on 3 consecutive days within 5 days in ICU were investigated. A subgroup analysis of 12 patients with, and 18 patients without CIM (non-CIM) was performed. Two consecutive biopsies from vastus lateralis were obtained at median days 5 and 15, early and late time points. Controls were 5 healthy subjects undergoing elective orthopedic surgery. A septic mouse model and cultured myoblasts were used for mechanistic analyses.Measurements and main resultsEarly SAA1 expression was significantly higher in skeletal muscle of CIM compared to non-CIM patients. Immunohistochemistry showed SAA1 accumulations in muscle of CIM patients at the early time point, which resolved later. SAA1 expression was induced by IL-6 and tumor necrosis factor-alpha in human and mouse myocytes in vitro. Inflammation-induced muscular SAA1 accumulation was reproduced in a sepsis mouse model.ConclusionsSkeletal muscle contributes to general inflammation and acute-phase response in CIM patients. Muscular SAA1 could be important for CIM pathogenesis.Trial registrationISRCTN77569430. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|