Unexpected features of exponentially growing Tobacco Bright Yellow-2 cell suspension culture in relation to excreted extracellular polysaccharides and cell wall composition
| Autor: | Celine Girard, Vincent Sol, Catherine Riou, Mohammad Issawi, Mohammad Muhieddine |
|---|---|
| Přispěvatelé: | Laboratoire de Chimie des Substances Naturelles (LCSN), Université de Limoges (UNILIM)-Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST FR CNRS 3503) |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine food.ingredient Pectin media_common.quotation_subject Cell Culture Techniques Uronic acid Biology 01 natural sciences Biochemistry Tobacco BY-2 suspension culture Excretion Cell wall 03 medical and health sciences chemistry.chemical_compound food Cell Wall Polysaccharides Plant Cells Tobacco Secretion [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Internalization Sugar Molecular Biology Cells Cultured media_common Extracellular polysaccharides [CHIM.ORGA]Chemical Sciences/Organic chemistry Cell wall composition Monosaccharides Cell Biology Hydrogen-Ion Concentration 030104 developmental biology Uronic Acids chemistry Pectins Composition (visual arts) 010606 plant biology & botany |
| Zdroj: | Glycoconjugate Journal Glycoconjugate Journal, Springer Verlag, 2017, 34 (5), pp.585-590. ⟨10.1007/s10719-017-9782-7⟩ |
| ISSN: | 1573-4986 0282-0080 |
| DOI: | 10.1007/s10719-017-9782-7⟩ |
| Popis: | International audience; This article presents a new insight about TBY-2 cells; from extracellular polysaccharides secretion to cell wall composition during cell suspension culture. In the medium of cells taken 2 days after dilution (end of lag phase), a two unit pH decrease from 5.38 to 3.45 was observed and linked to a high uronic acid (UA) amount secretion (47.8%) while, in 4 and 7 day-old spent media, pH increased and UA amounts decreased 35.6 and 42.3% UA, respectively. To attain deeper knowledge of the putative link between extracellular polysaccharide excretion and cell wall composition, we determined cell wall UA and neutral sugar composition of cells from D2 to D12 cultures. While cell walls from D2 and D3 cells contained a large amount of uronic acid (twice as much as the other analysed cell walls), similar amounts of neutral sugar were detected in cells from lag to end of exponential phase cells suggesting an enriched pectin network in young cultures. Indeed, monosaccharide composition analysis leads to an estimated percentage of pectins of 56% for D3 cell wall against 45% D7 cell walls indicating that the cells at the mid-exponential growth phase re-organized their cell wall linked to a decrease in secreted UA that finally led to a stabilization of the spent medium pH to 5.4. In conclusion, TBY-2 cell suspension from lag to stationary phase showed cell wall remodeling that could be of interest in drug interaction and internalization study. |
| Databáze: | OpenAIRE |
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