A stimulatory RNA associated with RecBCD enzyme
| Autor: | Susan K. Amundsen, Gerald R. Smith, Andrew F. Taylor |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Small RNA Exodeoxyribonuclease V RNA-dependent RNA polymerase chemistry.chemical_compound Transcription (biology) Genetics Polymerase RecBCD Recombination Genetic biology Sequence Analysis RNA DNA Helicases RNA Nicking enzyme Molecular biology RNA Bacterial Exodeoxyribonucleases chemistry Biochemistry Ribonucleoproteins biology.protein bacteria Nucleic Acid Conformation DNA Research Article |
| Zdroj: | Nucleic acids research. 26(9) |
| ISSN: | 0305-1048 |
| Popis: | RecBCD enzyme acts in the major pathway of homologous recombination of linear DNA in Escherichia coli. The enzyme unwinds DNA and is an ATP-dependent double-strand and single-strand exonuclease and a single-strand endonuclease; it acts at Chi recombination hotspots (5'-GCTGGTGG-3') to produce a recombinogenic single-stranded DNA 3'-end. We found that a small RNA with a unique sequence of approximately 24 nt was tightly bound to RecBCD enzyme and co-purified with it. When added to native enzyme this RNA, but not four others, increased DNA unwinding and Chi nicking activities of the enzyme. In seven similarly active enzyme preparations the molar ratio of RNA molecules to RecBCD enzyme molecules ranged from 0.2 to |
| Databáze: | OpenAIRE |
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