Antibodies to hepatitis B virus surface antigen and interleukin 12 and interleukin 18 gene polymorphisms in hemodialysis patients
| Autor: | Alicja E. Grzegorzewska, Adrianna Mostowska, Piotr Wobszal, Paweł P. Jagodziński |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty lcsh:RC870-923 Polymorphism Single Nucleotide Gastroenterology Interleukin 12 Renal Dialysis Internal medicine IL12A Genotype Prevalence Humans Medicine Genetic Predisposition to Disease Renal Insufficiency Hepatitis B Surface Antigens biology business.industry Interleukin-18 Gene polymorphisms Interleukin Antibodies to surface antigen of hepatitis B virus Interleukin 18 Odds ratio Middle Aged lcsh:Diseases of the genitourinary system. Urology Interleukin-12 Nephrology Hemodialysis Immunology biology.protein Female Poland Antibody Restriction fragment length polymorphism business Research Article |
| Zdroj: | BMC Nephrology BMC Nephrology, Vol 13, Iss 1, p 75 (2012) |
| ISSN: | 1471-2369 |
| DOI: | 10.1186/1471-2369-13-75 |
| Popis: | Background The interleukin (IL)18 rs360719 CC genotype is associated with the development of antibodies to hepatitis B virus surface antigen (anti-HBs) in hemodialysis (HD) patients. IL18 shares biological properties with IL12 in promoting the T-hepler 1 (Th1) system. We studied whether polymorphisms in the IL12A 3` untranslated region (UTR) and IL12B 3`UTR may contribute to anti-HBs development (titre ≥ 10 IU/L) in HD patients either individually or jointly with the IL18 polymorphism. Methods In 518 HD patients and 240 controls the IL12A rs568408 3’UTR G > A polymorphism was genotyped by high-resolution melting curve analysis. Polymerase chain reaction restriction fragment length polymorphism was used to detect the IL12B rs3212227 3’UTR A > C and IL18 -1297 T > C rs360719 polymorphisms. The associations between the IL12A, IL12B and IL18 genotypes and the risk of impaired anti-HBs development were estimated by computing the odds ratios and their 95% confidence intervals using logistic regression analysis. Results In the logistic regression analysis, the higher frequency of rs360719 CC individually (2.9% in 207 patients without anti-HBs development vs 8.0% in 311 patients with anti-HBs development, p = 0.009) and of rs360719 CC combined with rs568408 GG (p = 0.048), rs568408 GA (p = 0.035), rs568408 GG/AA (p = 0.034) or rs3212227 AA (p = 0.046) was associated with an increased chance for the development of anti-HBs in HD patients. Patients bearing both rs568408 AA and rs360719 TT had a 10.9-fold or 8.9-fold lower chance, respectively, to develop anti-HBs compared with those carrying any other genotype (p = 0.005) or those who had both wild-type rs568408 GG and rs360719 TT (p = 0.011). Carriers of both rs3212227 CC and rs360719 TC had a 4.6-fold lower chance for anti-HBs development than carriers of any other genotype (p = 0.042). Conclusion Development of anti-HBs in HD patients is associated with gene polymorphisms of interleukins involved in the Th1 system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink