Differential expression of L- and N-type voltage-sensitive calcium channels in the spinal cord of morphine+nimodipine treated rats
| Autor: | Yogendra Kumar Gupta, Subrata Basu Ray, Akhil Parashar, Dilip Verma |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Calcium Channels L-Type Calcium Channels N-Type Nifedipine Internal medicine Animals Medicine Rats Wistar Molecular Biology Nimodipine Analysis of Variance Morphine Voltage-dependent calcium channel business.industry General Neuroscience Calcium channel Morphine tolerance Drug Tolerance Spinal cord Immunohistochemistry Rats Posterior Horn Cells Endocrinology medicine.anatomical_structure Spinal Cord Anesthesia Neurology (clinical) business Developmental Biology medicine.drug |
| Zdroj: | Brain Research. 1249:128-134 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/j.brainres.2008.10.038 |
| Popis: | We have earlier reported that nifedipine and nimodipine, both L-type voltage-sensitive calcium channel (L-VSCC) antagonists, attenuate the development of tolerance to chronic administration of morphine in the rat. In the present study, we have investigated the expression of L- and N-type VSCC using immunohisto-chemistry, in the cervical region of the spinal cords from animals treated chronically with morphine alone or in combination with nimodipine. The highest expression of both VSCCs within the spinal cord was detected within the superficial laminae of the dorsal horn, which indicates that these channels play an important role in the spinal processing of pain. After morphine tolerance, the expression of both the channels in the superficial laminae was significantly higher than control animals. However, morphine + nimodipine administration produced a differential effect, that is, the expression of L-VSCC decreased while that of N-VSCC increased. The study shows that the expression of these channels is plastic and subject to change depending upon the drug administered. This in turn can determine overall responsiveness to morphine. |
| Databáze: | OpenAIRE |
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