Ischemic preconditioning limits infarct size following regional ischemia–reperfusion in in situ mouse hearts
| Autor: | D M Van Winkle, D L Miller |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Physiology Myocardial Infarction Ischemia Infarction Mice Inbred Strains Anterior Descending Coronary Artery Electrocardiography Mice chemistry.chemical_compound Reperfusion therapy Physiology (medical) Internal medicine medicine Animals medicine.diagnostic_test Tetrazolium chloride business.industry Myocardium medicine.disease Mice Inbred C57BL Microscopy Fluorescence chemistry Coronary occlusion Anesthesia Ischemic Preconditioning Myocardial Cardiology Ischemic preconditioning Female Cardiology and Cardiovascular Medicine business |
| Zdroj: | Cardiovascular Research. 42:680-684 |
| ISSN: | 0008-6363 |
| DOI: | 10.1016/s0008-6363(99)00005-x |
| Popis: | Objective: Ischemic preconditioning has been demonstrated in a wide variety of animals, from dogs to rats. Experimentally-induced murine myocardial ischemia–reperfusion has been described, but ischemic preconditioning has not been reported in mouse hearts. To test the hypothesis that mouse hearts exhibit preconditioning-induced protection, experiments were conducted in anesthetized open chest mice subjected to regional myocardial ischemia–reperfusion. Methods: Following barbiturate anesthesia the FVB and C57BL/6J mice underwent a tracheostomy and were mechanically ventilated. The heart was exposed via a left thoracotomy performed with the aid of a dissecting microscope. A 7-0 silk suture on a curved taper needle was passed under the proximal left anterior descending coronary artery to form a snare. Mice were then randomly assigned to receive either no preconditioning or preconditioning. All mice were subjected to 60 min regional myocardial ischemia followed by 2.5 h of reperfusion. Ischemic preconditioning (IP) was induced with two (FVB mice) or three (C57BL/6J mice) cycles of 5 min coronary occlusion and 5 min reperfusion. Control animals did not receive preconditioning ischemia. Area-at-risk was assessed with fluorescent particles. Infarct size was assessed with triphenyl tetrazolium chloride, and is expressed below as a percentage of the area-at-risk. Results: In FVB mice preconditioning reduced infarct size 49%, from 36.7±4.5% to 18.9±2.8% ( P |
| Databáze: | OpenAIRE |
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