Non-ribosomal Peptide Synthases from Pseudomonas aeruginosa Play a Role in Cyclodipeptide Biosynthesis, Quorum-Sensing Regulation, and Root Development in a Plant Host
| Autor: | Omar González, José López-Bucio, Jesús Campos-García, Viridiana Magaña-Dueñas, Alma Laura Díaz-Pérez, Randy Ortiz-Castro, César Díaz-Pérez |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Indoles Virulence Factors 030106 microbiology Mutant Arabidopsis Soil Science Virulence medicine.disease_cause Peptides Cyclic Plant Roots Piperazines 03 medical and health sciences chemistry.chemical_compound Pyocyanin Biosynthesis medicine Arabidopsis thaliana Ecology Evolution Behavior and Systematics Ecology biology Indoleacetic Acids Pseudomonas aeruginosa food and beverages Quorum Sensing Dipeptides Gene Expression Regulation Bacterial biology.organism_classification Quorum sensing 030104 developmental biology chemistry Biochemistry Peptide Biosynthesis Nucleic Acid-Independent Pyocyanine Bacteria Signal Transduction |
| Zdroj: | Microbial ecology. 73(3) |
| ISSN: | 1432-184X |
| Popis: | Diverse molecules mediate cross-kingdom communication between bacteria and their eukaryotic partners and determine pathogenic or symbiotic relationships. N-acyl-L-homoserine lactone-dependent quorum-sensing signaling represses the biosynthesis of bacterial cyclodipeptides (CDPs) that act as auxin signal mimics in the host plant Arabidopsis thaliana. In this work, we performed bioinformatics, biochemical, and plant growth analyses to identify non-ribosomal peptide synthase (NRPS) proteins of Pseudomonas aeruginosa, which are involved in CDP synthesis. A reverse genetics strategy allowed the identification of the genes encoding putative multi-modular-NRPS (MM-NRPS). Mutations in these genes affected the synthesis of the CDPs cyclo(L-Pro-L-Val), cyclo(L-Pro-L-Leu), and cyclo(L-Pro-L-Tyr), while showing wild-type-like levels of virulence factors, such as violacein, elastase, and pyocyanin. When analyzing the bioactivity of purified, naturally produced CDPs, it was found that cyclo(L-Pro-L-Tyr) and cyclo(L-Pro-L-Val) were capable of antagonizing quorum-sensing-LasR (QS-LasR)-dependent signaling in a contrasting manner in the cell-free supernatants of the selected NRPS mutants, which showed QS induction. Using a bacteria-plant interaction system, we further show that the pvdJ, ambB, and pchE P. aeruginosa mutants failed to repress primary root growth, but improved root branching in A. thaliana seedlings. These results indicated that the CDP production in P. aeruginosa depended on the functional MM-NRPS, which influences quorum-sensing of bacteria and plays a role in root architecture remodeling. |
| Databáze: | OpenAIRE |
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