Expression and functional analysis of the cellular retinoic acid binding protein from silkworm pupae (Bombyx mori)
| Autor: | Hongdan Shen, Jian Chen, Xuedong Wang, Yaozhou Zhang, Zuoming Nie, Xue-Jian Wang, Dan Wang, Zhengbing Lv, Xiang-Fu Wu |
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| Rok vydání: | 2007 |
| Předmět: |
Receptors
Retinoic Acid Drug Resistance Retinoic acid Tretinoin Biology Biochemistry Cell Line Retinoic acid-inducible orphan G protein-coupled receptor chemistry.chemical_compound Bombyx mori Animals MTT assay Cloning Molecular neoplasms Molecular Biology Cell Proliferation Bombyx organic chemicals fungi Pupa Cell Biology biology.organism_classification Subcellular localization Molecular biology biological factors Retinoic acid receptor Gene Expression Regulation chemistry Growth inhibition |
| Zdroj: | Journal of Cellular Biochemistry. 102:970-979 |
| ISSN: | 1097-4644 0730-2312 |
| DOI: | 10.1002/jcb.21333 |
| Popis: | Cellular retinoic acid binding protein (CRABP) is a member of intracellular lipid-binding protein (iLBP), and closely associated with retinoic acid (RA) activity. We have cloned the CRABP gene from silkworm pupae and studied the interaction between Bombyx mori CRABP (BmCRABP) and all-trans retinoic acid (atRA). The MTT assay data indicated that when BmCRABP is overexpressed in Bm5 cells, the cells dramatically resisted to atRA-induced growth inhibition. Conversely, the cells were sensitive to atRA treatment upon knocking down the BmCRABP expression. Subcellular localization revealed that BmCRABP is a cytoplasm protein, even when treated with atRA, the CRABP still remained in the cytoplasm. These data demonstrated that the function of BmCRABP have an effect on the physiological function of atRA. |
| Databáze: | OpenAIRE |
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