Additional file 1: of Impact of poly(A)-tail G-content on Arabidopsis PAB binding and their role in enhancing translational efficiency

Autor: Taolan Zhao, Huan, Qing, Sun, Jing, Chunyan Liu, Xiuli Hou, Yu, Xiang, Silverman, Ian, Zhang, Yi, Gregory, Brian, Liu, Chun-Ming, Wenfeng Qian, Xiaofeng Cao
Rok vydání: 2019
DOI: 10.6084/m9.figshare.9766316
Popis: Figure S1. AtPAB2, AtPAB4, and AtPAB8 are constitutively expressed in Arabidopsis. Figure S2. The mutants of AtPABs exhibited severe phenotypes. Figure S3. The expression of AtPAB-GFP-HA rescued the developmental defects of atpab double mutants. Figure S4. AtPAB-GFP-HA reporters were ubiquitously and constitutively expressed in the cytoplasm during seed development. Figure S5. Identification of AtPAB-binding targets. Figure S6. The AtPAB-binding genes detected in CLIP-seq were validated by RIP-RT-PCR. Figure S7. AtPABs bind the poly(A) tails of mRNAs with different efficiencies. Figure S8. G% in the poly(A) tail is a determinant of the AtPAB-binding efficiency. Figure S9. The major consequence of AtPAB-binding is translational enhancement. Figure S10. AtPAB binding enhances translation efficiency. (PDF 1987 kb)
Databáze: OpenAIRE