Tricomponent composite containing copper-hydroxyapatite/chitosan/polyvinyl pyrrolidone for bone tissue engineering
| Autor: | Valarmathi Narayanan, Shanmugam Sumathi, Arunai Nambi Raj Narayanasamy |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
Biocompatibility Simulated body fluid 0206 medical engineering Composite number Biomedical Engineering Biocompatible Materials 02 engineering and technology Apatite Bone and Bones Cell Line Biomaterials Chitosan chemistry.chemical_compound Mice stomatognathic system Anti-Infective Agents Ultimate tensile strength medicine Animals Humans Cell Proliferation Osteoblasts Tissue Engineering Tissue Scaffolds technology industry and agriculture Metals and Alloys Povidone 021001 nanoscience & nanotechnology 020601 biomedical engineering Solvent Durapatite chemistry visual_art Ceramics and Composites visual_art.visual_art_medium Swelling medicine.symptom 0210 nano-technology Copper Nuclear chemistry |
| Zdroj: | Journal of biomedical materials research. Part AREFERENCES. 108(9) |
| ISSN: | 1552-4965 |
| Popis: | The tricomponent composite chitosan/polyvinyl pyrrolidone (CS/PVP) with different weight ratios of (0, 20, 40, 60, and 80 wt%) of copper-hydroxyapatite (Cu-HAP) were prepared by solvent casting technique, which was characterized by X-ray diffraction, Fourier-transform infrared, and scanning electron microscopy with energy-dispersive X-ray to confirm the formation of Cu-HAP/CS/PVP composite. The Cu-HAP/CS/PVP composite with 80 wt% Cu-HAP showed 98.73 ± 1.14% of porosity with highest tensile strength (101.45 ± 0.98 MPa) and less swelling percentage (19.51 ± 1.03%) compared to others. In vitro antimicrobial activity was investigated against bacteria (Staphylococcus aureus, Bacillus subtilis, and Escherichia coli) and fungus (Candida albicans, Penicillium notatum, and Rhizopus stolonifer). In vitro hemocompatibility study proves that the Cu-HAP/CS/PVP composites are blood compatible with the hemolytic ratio of less than 2%. In vitro bioactivity study revealed the formation of apatite on the optimized Cu-HAP/CS/PVP composite (80 wt% of Cu-HAP) in simulated body fluid (SBF) solution. ICP-OES (Inductively Coupled Plasma-Optical Emission Spectroscopy) analysis was used to find out the leaching of Ca, P, and Cu ions from the SBF. In vitro biocompatibility was studied against human osteosarcoma cell line by MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-DiphenyltetrazoliumBromide) assay. |
| Databáze: | OpenAIRE |
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