Resolution of diastereomers of N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine (quadrol) by reversed-phase high-performance liquid chromatography
| Autor: | Daniel J. Smith, J.A. Fulton, H.K. Pokharna, Michael J. Dunphy |
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| Rok vydání: | 1988 |
| Předmět: |
Chromatography
Magnetic Resonance Spectroscopy Resolution (mass spectrometry) Organic Chemistry Diastereomer Ethylenediamine Stereoisomerism Biological activity General Medicine Nuclear magnetic resonance spectroscopy Ethylenediamines Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound chemistry Adjuvants Immunologic Propylene oxide Chromatography High Pressure Liquid |
| Zdroj: | Journal of chromatography. 455 |
| Popis: | Quadrol, N,N,N',N'-tetrakis(2-hydroxypropyl)ethylenediamine, has been recently observed to display biological activity. It is an immunostimulant and has been implicated as a potentially useful agent in accelerated wound healing. Quadrol exists as a mixture of four unique diastereomers, each of which may, upon further investigation, display differences in biological activity. This paper describes an high-performance liquid chromatographic procedure (both analytical and prep) for the separation of the Quadrol diastereomers. Gas-liquid chromatography and NMR data are presented which corroborate the high-performance liquid chromatographic results. This procedure may be used to obtain pure Quadrol diastereomers, to monitor the progress of Quadrol synthesis from propylene oxide and ethylenediamine or to develop a quantitative assay for Quadrol diastereomers. |
| Databáze: | OpenAIRE |
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