Adenosine Activates Aromatic L-Amino Acid Decarboxylase Activity in the Kidney and Increases Dopamine
| Autor: | Takanobu Takezako, Manabu Koga, Keita Noda, Emiko Tsuji, Kikuo Arakawa, Manabu Sasaguri |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
medicine.medical_specialty Adenosine Swine Dopamine Natriuresis Kidney Dopamine agonist Levodopa Rats Sprague-Dawley Adenosine A1 receptor Adenosine deaminase Dopamine receptor D1 Internal medicine Purinergic P1 Receptor Agonists medicine Animals Aromatic L-amino acid decarboxylase biology Chemistry Receptors Dopamine D1 Kidney metabolism General Medicine Benzazepines Diuresis Rats Enzyme Activation medicine.anatomical_structure Endocrinology Purinergic P1 Receptor Antagonists Aromatic-L-Amino-Acid Decarboxylases Nephrology biology.protein LLC-PK1 Cells medicine.drug |
| Zdroj: | Journal of the American Society of Nephrology. 12:29-36 |
| ISSN: | 1046-6673 |
| DOI: | 10.1681/asn.v12129 |
| Popis: | Renal sodium handling is important for regulating BP, and renal dopamine and adenosine play an important role in renal sodium handling, however the interaction of these hormones in the kidney was not clarified. In in vivo experiments, adenosine significantly increased water and sodium excretion by 50% compared with vehicle when infused into the left renal artery, accompanied by an increase in urinary dopamine excretion in the left kidney. Neither water-sodium excretion nor dopamine excretion changed in the vehicle-infused kidney. Aromatic L-amino acid decarboxylase activity in the left kidney was significantly higher than that in the noninfused right kidney. The increase in water-sodium excretion induced by adenosine was significantly inhibited by SCH23390, a selective D1 receptor antagonist. In in vitro experiments, porcine renal proximal tubular cells were incubated with 250 microM L-dopa and N(6)-cyclohexyladenosine, an adenosine type 1 receptor agonist, after treatment with adenosine deaminase. N(6)-cyclohexyladenosine significantly increased dopamine formation at a concentration of 10(-9) to 10(-7) M, and this was completely inhibited by 1,3-dipropyl-8-cyclopentylxanthin, an adenosine A1 antagonist. These results show that renal dopamine synthesis is stimulated by adenosine through the activation of aromatic L-amino acid decarboxylase and suggest that adenosine leads to an increase in renal dopamine and natriuresis. |
| Databáze: | OpenAIRE |
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