HIT family genes: FHIT but not PKCI-1/HINT produces altered transcripts in colorectal cancer
| Autor: | Elnatan J, David Murphy, H S Goh, Duncan R. Smith |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Cancer Research
HIT genes Transcription Genetic Tumor suppressor gene Hydrolases Molecular Sequence Data Nerve Tissue Proteins Adenocarcinoma Biology medicine.disease_cause Exon FHIT medicine Humans Gene colorectal Genetics Base Sequence Reverse Transcriptase Polymerase Chain Reaction tumour Point mutation Alternative splicing Proteins Regular Article Molecular biology Reverse transcriptase Acid Anhydride Hydrolases Neoplasm Proteins Oncology Protein Biosynthesis Colorectal Neoplasms transcription Carcinogenesis |
| Zdroj: | University of Bristol-PURE British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6690779 |
| Popis: | Forty-five colorectal adenocarcinomas were examined for alterations in the HIT family genes FHIT and PKCI-1/HINT by a combination of reverse transcriptase polymerase chain reaction and DNA sequencing. In all cases a single transcript corresponding to the reported sequence was detected using primers specific for the PKCI-1/HINT gene. In contrast multiple transcripts were detected using primers specific for the FHIT gene transcript. 6% (3/45) of tumours evinced no detectable expression of any FHIT transcript and a further 12% (6/45) produced only the normal full length transcripts. Ninety-six aberrant transcripts were characterized from the remaining tumours. Deviations from the normal full length sequence characterized included deletions, insertions of novel sequences, a point mutation as well as the usage of a putative alternate splice site in exon 10. Message variants were detected with approximately equal frequency in all tumour stages with the exception that templates with insertions were found solely in Dukes’ stage B tumours (P < 0.001). With the exception of the putative alternate splice site, aberrant transcripts were not detected in matched normal mucosa. These results suggest that members of the HIT family of genes are only selectively involved in tumorigenesis and that perturbation of FHIT gene expression is an early event in colorectal tumorigenesis. © 1999 Cancer Research Campaign |
| Databáze: | OpenAIRE |
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