Development of 3D Printed Bruch’s Membrane-Mimetic Substance for the Maturation of Retinal Pigment Epithelial Cells

Autor: Dong-Woo Cho, Hyungseok Lee, Jeong Sik Kong, Jong Min Kim, Ju Young Park, Jae Yon Won
Jazyk: angličtina
Rok vydání: 2021
Předmět:
0301 basic medicine
Retinal degeneration
tissue-specific bioink
genetic structures
Swine
retinal pigment epithelium
Angiogenesis Inhibitors
Bruch's membrane
lcsh:Chemistry
chemistry.chemical_compound
Macular Degeneration
0302 clinical medicine
Laminin
Biomimetics
lcsh:QH301-705.5
Spectroscopy
biology
Microvilli
General Medicine
Computer Science Applications
Cell biology
Extracellular Matrix
medicine.anatomical_structure
Printing
Three-Dimensional
Rheology
Visual phototransduction
in vitro RPE model
RPE maturation
In Vitro Techniques
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Phagocytosis
Retinitis pigmentosa
medicine
Cell Adhesion
Animals
Physical and Theoretical Chemistry
Molecular Biology
Cell Proliferation
tissue-mimetic substrate
Retinal pigment epithelium
Organic Chemistry
Retinal
medicine.disease
eye diseases
Rats
Stargardt disease
Disease Models
Animal
030104 developmental biology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
030221 ophthalmology & optometry
biology.protein
Bruch Membrane
sense organs
Zdroj: International Journal of Molecular Sciences
Volume 22
Issue 3
International Journal of Molecular Sciences, Vol 22, Iss 1095, p 1095 (2021)
ISSN: 1422-0067
DOI: 10.3390/ijms22031095
Popis: Retinal pigment epithelium (RPE) is a monolayer of the pigmented cells that lies on the thin extracellular matrix called Bruch&rsquo
s membrane. This monolayer is the main component of the outer blood&ndash
retinal barrier (BRB), which plays a multifunctional role. Due to their crucial roles, the damage of this epithelium causes a wide range of diseases related to retinal degeneration including age-related macular degeneration, retinitis pigmentosa, and Stargardt disease. Unfortunately, there is presently no cure for these diseases. Clinically implantable RPE for humans is under development, and there is no practical examination platform for drug development. Here, we developed porcine Bruch&rsquo
s membrane-derived bioink (BM-ECM). Compared to conventional laminin, the RPE cells on BM-ECM showed enhanced functionality of RPE. Furthermore, we developed the Bruch&rsquo
s membrane-mimetic substrate (BMS) via the integration of BM-ECM and 3D printing technology, which revealed structure and extracellular matrix components similar to those of natural Bruch&rsquo
s membrane. The developed BMS facilitated the appropriate functions of RPE, including barrier and clearance functions, the secretion of anti-angiogenic growth factors, and enzyme formation for phototransduction. Moreover, it could be used as a basement frame for RPE transplantation. We established BMS using 3D printing technology to grow RPE cells with functions that could be used for an in vitro model and RPE transplantation.
Databáze: OpenAIRE
Externí odkaz: