Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination
| Autor: | Paulina Ampomah, Lars Hviid, Mads Delbo Larsen, Morten Nielsen, Emmanuel K. Dickson, Jan Nouta, Nicaise Tuikue Ndam, Carolien A. M. Koeleman, Ali Salanti, Benjamin Mordmüller, Agnes L. Hipgrave Ederveen, Michael F. Ofori, Achille Massougbodji, Mary Lopez-Perez, Manfred Wuhrer, Gestur Vidarsson, C. Ellen van der Schoot |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Protein vaccines
Protein subunit Phagocytosis Science Plasmodium falciparum Glycobiology lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4] General Physics and Astronomy Antibodies Protozoan Antigens Protozoan General Biochemistry Genetics and Molecular Biology Article Antibodies Microbiology All institutes and research themes of the Radboud University Medical Center Antigen Viral envelope Immunity Pregnancy parasitic diseases Humans Malaria Falciparum Receptor Multidisciplinary biology Chemistry Vaccination General Chemistry biology.organism_classification Malaria Membrane protein Immunoglobulin G Immunology embryonic structures biology.protein Female Antibody Parasite host response |
| Zdroj: | Nature Communications, 12, 1 Nature Communications, 12 Nature Communications, 12(1). NATURE PORTFOLIO Nature Communications Larsen, M D, Lopez-Perez, M, Dickson, E K, Ampomah, P, Tuikue Ndam, N, Nouta, J, Koeleman, C A M, Ederveen, A L H, Mordmüller, B, Salanti, A, Nielsen, M A, Massougbodji, A, van der Schoot, C E, Ofori, M F, Wuhrer, M, Hviid, L & Vidarsson, G 2021, ' Afucosylated Plasmodium falciparum-specific IgG is induced by infection but not by subunit vaccination ', Nature Communications, vol. 12, 5838 . https://doi.org/10.1038/s41467-021-26118-w Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021) Nature Communications, 12, 1. Nature Publishing Group |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-021-26118-w |
| Popis: | Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family members mediate receptor- and tissue-specific sequestration of infected erythrocytes (IEs) in malaria. Antibody responses are a central component of naturally acquired malaria immunity. PfEMP1-specific IgG likely protects by inhibiting IE sequestration and through IgG-Fc Receptor (FcγR) mediated phagocytosis and killing of antibody-opsonized IEs. The affinity of afucosylated IgG to FcγRIIIa is up to 40-fold higher than fucosylated IgG, resulting in enhanced antibody-dependent cellular cytotoxicity. Most IgG in plasma is fully fucosylated, but afucosylated IgG is elicited in response to enveloped viruses and to paternal alloantigens during pregnancy. Here we show that naturally acquired PfEMP1-specific IgG is strongly afucosylated in a stable and exposure-dependent manner, and efficiently induces FcγRIIIa-dependent natural killer (NK) cell degranulation. In contrast, immunization with a subunit PfEMP1 (VAR2CSA) vaccine results in fully fucosylated specific IgG. These results have implications for understanding protective natural- and vaccine-induced immunity to malaria. Here, Larsen et al. describe differences in Fc fucosylation of P. falciparum PfEMP1-specific IgG produced in response to natural infection versus VAR2CSA-type subunit vaccination, which leads to differences in the ability to induce FcγRIIIa-dependent natural killer cell degranulation. |
| Databáze: | OpenAIRE |
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