Role of X-linked inhibitor of apoptosis-associated factor-1 in vasculogenic mimicry in ovarian cancer

Autor: Yunxia Wang, Xietong Wang, Hongluan Mao, Peishu Liu
Rok vydání: 2017
Předmět:
0301 basic medicine
Cancer Research
endocrine system diseases
Gene Expression
Biochemistry
Metastasis
Mice
0302 clinical medicine
Cell Movement
Ovarian carcinoma
Ovarian Neoplasms
Neovascularization
Pathologic
Intracellular Signaling Peptides and Proteins
Middle Aged
Immunohistochemistry
female genital diseases and pregnancy complications
Neoplasm Proteins
XIAP
Platelet Endothelial Cell Adhesion Molecule-1
Oncology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Heterografts
Molecular Medicine
Female
Adult
X-Linked Inhibitor of Apoptosis Protein
Biology
Inhibitor of apoptosis
03 medical and health sciences
Cell Line
Tumor
Genetics
medicine
Animals
Humans
Vasculogenic mimicry
Molecular Biology
Adaptor Proteins
Signal Transducing
Aged
Neoplasm Staging
Oncogene
Cancer
medicine.disease
Disease Models
Animal
030104 developmental biology
Cancer research
Neoplasm Grading
Apoptosis Regulatory Proteins
Ovarian cancer
Biomarkers
Zdroj: Molecular Medicine Reports. 16:325-330
ISSN: 1791-3004
1791-2997
Popis: X-linked inhibitor of apoptosis‑associated factor 1 (XAF1) was identified as a novel X-linked inhibitor of apoptosis (XIAP) binding partner that may reverse the anti‑apoptotic effect of XIAP. Previous studies have revealed that XAF1 serves an important role in cancer angiogenesis. Vasculogenic mimicry (VM) describes the formation of fluid‑conducting channels by highly invasive and genetically dysregulated tumor cells. VM is critical for tumor blood supply and is associated with aggressive actions and metastasis. The aim of present study was to investigate the potential association between XAF1 expression with VM of ovarian cancer, and evaluate the role of XAF1 in tumor cell migration and invasion of SKOV3 cells. VM structure and XAF1 expression were detected in 94 tissue samples of advanced epithelial ovarian cancer (EOC). Invasion and migration of the SKOV3 human ovarian carcinoma cell line were identified by Transwell assay. It was revealed that the presence of VM was associated with high grade advanced ovarian cancer. Reduced XAF1 expression was significantly associated with presence of VM. Overexpression of XAF1 significantly reduced invasion and migration of SKOV3 cells, and inhibited vascular endothelial growth factor protein expression. Furthermore, vasculature was suppressed by overexpression of XAF1 in vivo in xenograft models. In conclusion, XAF1 expression was associated with VM in ovarian cancer, suggesting a potential role of XAF1 in the formation of VM in EOC. These findings may facilitate the development of novel therapeutic agents for the treatment of ovarian cancer.
Databáze: OpenAIRE
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