Anaerobic induction of the maize GapC4 promoter in poplar leaves requires light and high CO2
| Autor: | Robert Hänsch, Jutta Schulze, Rüdiger Cerff, Ralf R. Mendel, Reinhard Hehl, Tobias Kurz |
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| Rok vydání: | 2003 |
| Předmět: |
Light
Nicotiana tabacum Transgene Plant Science Zea mays Oxygen Consumption Salicaceae Botany Gene expression Genetics Arabidopsis thaliana Anaerobiosis Promoter Regions Genetic Gene Glucuronidase Plant Proteins Reporter gene biology fungi food and beverages Glyceraldehyde-3-Phosphate Dehydrogenases Carbon Dioxide biology.organism_classification Plants Genetically Modified Aerobiosis Isoenzymes Plant Leaves Populus Solanum |
| Zdroj: | Planta. 218(1) |
| ISSN: | 0032-0935 |
| Popis: | The maize (Zea mays L.) glyceraldehyde-3-phosphate dehydrogenase gene 4 ( GapC4) promoter confers anaerobic gene expression in tobacco (Nicotiana tabacum L.), potato (Solanum tuberosum L.) and Arabidopsis thaliana (L.) Heynh. Here we have investigated its expression in hybrid poplar (Populus tremula x P. alba). Our results show that the promoter is not expressed in leaves and stems under normoxic conditions while anaerobiosis induces reporter gene expression in leaves up to a level observed for the STLS-1 promoter from potato that is shown to confer leaf-specific gene expression in transgenic poplar. Anaerobic induction is cell autonomous and requires a CO2 atmosphere and light. As in tobacco, the GapC4 promoter in poplar is wound inducible. The induction by CO2 and light may reflect a natural situation because flooding, a natural cause of anaerobiosis, is often accompanied by high CO2 concentrations in the floodwater. Our results show that the GapC4 promoter is suitable as an anaerobic reporter and as an inducible gene expression system in poplar. |
| Databáze: | OpenAIRE |
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