Differential expression of cell-cell junction proteins in the testis, epididymis, and ductus deferens of domestic turkeys (Meleagris gallopavo) with white and yellow semen
| Autor: | Barbara Bilińska, Malgorzata Brzoskwinia, Jan Jankowski, Andrzej Ciereszko, Alicja Kamińska, Laura Pardyak, Anna Hejmej, Malgorzata Kotula-Balak |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
Turkeys Cell-cell junction Physiology and Reproduction Population Connexin Gene Expression Semen Biology Occludin Andrology Avian Proteins 03 medical and health sciences Western blot Gene expression medicine Animals education yellow semen syndrome lcsh:SF1-1100 030304 developmental biology 0303 health sciences education.field_of_study Sertoli Cells Tight Junction Proteins medicine.diagnostic_test 0402 animal and dairy science Epithelial Cells 04 agricultural and veterinary sciences General Medicine Epididymis cell-cell junctional proteins 040201 dairy & animal science medicine.anatomical_structure Germ Cells Animal Science and Zoology lcsh:Animal culture testis-epididymis-ductus deferens domestic turkey |
| Zdroj: | Poultry Science Poultry Science, Vol 99, Iss 1, Pp 555-566 (2020) |
| ISSN: | 1525-3171 |
| Popis: | Tight, adherens, and gap junctions are involved in the regulation of reproductive tissue function in male mammals. In birds, including domestic turkeys, intercellular interactions performed by junctional networks have not yet been studied. Furthermore, the cellular and molecular basis of yellow semen syndrome (YSS) in the turkey population remains poorly understood. Thus, the aim of the present study was 2-fold: first, to provide new information on the localization and expression of cell-cell junction proteins in the testis, epididymis, and ductus deferens of domestic turkeys and second, to compare expression of junctional protein genes between 2 turkey population, one that produces white normal semen (WNS) and the other that produces yellow abnormal semen. Expression of occludin, zonula occludens-1 (ZO-1), connexin 43 (Cx43), N- and E-cadherin, and β-catenin genes were investigated using 3 complementary techniques: quantitative real-time PCR, western blot, and immunohistochemistry. Compared to WNS testis, epididymis, and ductus deferens, YSS tissues exhibited downregulation of occludin and β-catenin mRNA (P < 0.05) and protein (P < 0.05 and P < 0.01, respectively) and upregulation of N- and E-cadherin mRNA (P < 0.001, P < 0.05, P < 0.01, respectively) and protein (P < 0.01, P < 0.05, and P < 0.05, respectively). In contrast, ZO-1 and Cx43 mRNA and protein were upregulated in YSS testis (P < 0.05 and P < 0.001, respectively) but not in epididymis and ductus deferens; both mRNAs and proteins were downregulated (P < 0.05) compared to the respective WNS epididymis and ductus deferens. Altered staining intensity of immunoreactive proteins in YSS vs. WNS reproductive tissue sections confirmed the gene expression results. The present study is the first to demonstrate altered levels of junctional protein gene expression in reproductive tissues of male YSS turkeys. These findings may suggest that subtle changes in junctional protein expression affect the microenvironment in which spermatozoa develop and mature and thus may have an impact on the appearance of yellow semen in domestic turkeys. |
| Databáze: | OpenAIRE |
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