Expressions of hepatic genes, especially IGF-binding protein-1, correlating with serum corticosterone in microarray analysis
| Autor: | N L Lum, N K Bhat, Robert Y.S. Cheng, W G Alvord, Lucy M. Anderson, Douglas A Powell, Kazimierz S. Kasprzak, L A Birely, C M Perella, J M Cherry |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Microarray Biology chemistry.chemical_compound Mice Endocrinology Tyrosine aminotransferase Corticosterone Internal medicine Complementary DNA Gene expression medicine Animals Molecular Biology Heat-Shock Proteins Oligonucleotide Array Sequence Analysis Tyrosine Transaminase Microarray analysis techniques Gene Expression Profiling Nuclear Proteins Pleiotropic regulator 1 Molecular biology Gene Expression Regulation Neoplastic Insulin-Like Growth Factor Binding Protein 1 chemistry Liver Gene chip analysis Female Apoptosis Regulatory Proteins Carrier Proteins |
| Zdroj: | Journal of molecular endocrinology. 32(1) |
| ISSN: | 0952-5041 |
| Popis: | Microarray technology was evaluated for usefulness in assessing relationships between serum corticosterone and hepatic gene expression. Nine pairs of female Swiss mice were chosen to provide a wide range of serum corticosterone ratios; cDNA microarray analysis (approximately 8000 genes) was performed on their livers. A statistical method based on calculation of 99% confidence intervals discovered 32 genes which varied significantly among the livers. Five of these ratios correlated significantly with serum corticosterone ratio, including tyrosine aminotransferase, stress-induced protein, pleiotropic regulator 1 and insulin-like growth factor-binding protein-1; the latter has a potential role in cancer development. Secondly, linear regression of gene expression vs corticosterone ratios was screened for those with r> or =0.8 (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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