Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes
| Autor: | Akemi Kawasaki, Yasumasa Ishida, Hiroyuki Nishimura, Tasuku Honjo, Yasutoshi Agata, Takeshi Tsubata, Hideo Yagita |
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| Rok vydání: | 1996 |
| Předmět: |
T-Lymphocytes
T cell Lymphocyte Programmed Cell Death 1 Receptor Immunology Lymphocyte Activation Cell Line TCIRG1 Mice Cricetulus Antigen Cricetinae medicine Animals Immunology and Allergy Cytotoxic T cell Antigen-presenting cell B-Lymphocytes biology Antibodies Monoclonal Proteins General Medicine T lymphocyte Flow Cytometry Precipitin Tests Molecular biology Neoplasm Proteins Mice Inbred C57BL medicine.anatomical_structure Antigens Surface biology.protein Female Antibody Apoptosis Regulatory Proteins |
| Zdroj: | International Immunology. 8:765-772 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/8.5.765 |
| Popis: | A mAb J43 has been produced against the product of the mouse PD-1 gene, a member of the Ig gene superfamily, which was previously isolated from an apoptosis-induced T cell hybridoma (2B4.11) by using subtractive hybridization. Analyses by flow cytometry and immunoprecipitation using the J43 mAb revealed that the PD-1 gene product is a 50-55 kDa membrane protein expressed on the cell surface of several PD-1 cDNA transfectants and 2B4.11 cells. Since the molecular weight calculated from the amino acid sequence is 29, 310, the PD-1 protein appears to be heavily glycosylated. Normal murine lymphoid tissues such as thymus, spleen, lymph node and bone marrow contained very small numbers of PD-1(+) cells. However, a significant PD-1(+) population appeared in the thymocytes as well as T cells in spleen and lymph nodes by the in vivo anti-CD3 mAb treatment. Furthermore, the PD-1 antigen expression was strongly induced in distinct subsets of thymocytes and spleen T cells by in vitro stimulation with either anti-CD3 mAb or concanavalin A (Con A) which could lead T cells to both activation and cell death. Similarly, PD-1 expression was induced on spleen B cells by in vitro stimulation with anti-IgM antibody. By contrast, PD-1 was not significantly expressed on lymphocytes by treatment with growth factor deprivation, dexamethasone or lipopolysaccharide. These results suggest that the expression of the PD-1 antigen is tightly regulated and induced by signal transduction through the antigen receptor and do not exclude the possibility that the PD-1 antigen may play a role in clonal selection of lymphocytes although PD-1 expression is not required for the common pathway of apoptosis. |
| Databáze: | OpenAIRE |
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