Bone marrow-derived mesenchymal stem cells propagate immunosuppressive/anti-inflammatory macrophages in cell-to-cell contact-independent and -dependent manners under hypoxic culture
| Autor: | Masato Ohtsuka, Naoto Okubo, Takashi Yaegashi, Masaharu Kamo, Toshinari Mikami, Akira Ishisaki, Keita Suzuki, Miho Ibi, Seiko Kyakumoto, Masayuki Taira, Naoyuki Chosa, Seiji Yokota, Naoki Takizawa |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lymphocyte medicine.medical_treatment Anti-Inflammatory Agents Vascular Cell Adhesion Molecule-1 Cell Communication Biology Mice 03 medical and health sciences Bone Marrow medicine Animals Macrophage Cell adhesion Cells Cultured Macrophages Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Cell Biology Macrophage Activation M2 Macrophage Intercellular adhesion molecule Cell Hypoxia Coculture Techniques 030104 developmental biology medicine.anatomical_structure Cytokine Immunology Cancer research Bone marrow |
| Zdroj: | Experimental Cell Research. 358:411-420 |
| ISSN: | 0014-4827 |
| Popis: | Immunosuppressive/anti-inflammatory macrophage (Mφ), M2-Mφ that expressed the typical M2-Mφs marker, CD206, and anti-inflammatory cytokine, interleukin (IL)-10, is beneficial and expected tool for the cytotherapy against inflammatory diseases. Here, we demonstrated that bone marrow-derived lineage-positive (Lin+) blood cells proliferated and differentiated into M2-Mφs by cooperation with the bone marrow-derived mesenchymal stem cells (MSCs) under hypoxic condition: MSCs not only promoted proliferation of undifferentiated M2-Mφs, pre-M2-Mφs, in the Lin+ fraction via a proliferative effect of the MSCs-secreted macrophage colony-stimulating factor, but also promoted M2-Mφ polarization of the pre-M2-Mφs through cell-to-cell contact with the pre-M2-Mφs. Intriguingly, an inhibitor for intercellular adhesion molecule (ICAM)-1 receptor/lymphocyte function-associated antigen (LFA)-1, Rwj50271, partially suppressed expression of CD206 in the Lin+ blood cells but an inhibitor for VCAM-1 receptor/VLA-4, BIO5192, did not, suggesting that the cell-to-cell adhesion through LFA-1 on pre-M2-Mφs and ICAM-1 on MSCs was supposed to promoted the M2-Mφ polarization. Thus, the co-culture system consisting of bone marrow-derived Lin+ blood cells and MSCs under hypoxic condition was a beneficial supplier of a number of M2-Mφs, which could be clinically applicable to inflammatory diseases. |
| Databáze: | OpenAIRE |
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