LINC-PINT Suppresses the Aggressiveness of Thyroid Cancer by Downregulating miR-767-5p to Induce TET2 Expression
| Autor: | Jiaxiang Wang, Xiubo Lu, Zhuyao Li, Meng Jia, Mengjiao Pan, Mei Tao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Colorectal cancer LINC-PINT growth miR-767-5p medicine.disease_cause law.invention Metastasis 03 medical and health sciences 0302 clinical medicine law Drug Discovery microRNA medicine thyroid cancer Thyroid cancer business.industry lcsh:RM1-950 medicine.disease invasion Long non-coding RNA lcsh:Therapeutics. Pharmacology 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Molecular Medicine Suppressor Ectopic expression Original Article Carcinogenesis business |
| Zdroj: | Molecular Therapy. Nucleic Acids Molecular Therapy: Nucleic Acids, Vol 22, Iss, Pp 319-328 (2020) |
| ISSN: | 2162-2531 |
| Popis: | Long noncoding RNA (lncRNA) long intergenic nonprotein-coding RNA, p53-induced transcript (LINC-PINT) has shown anti-invasive activity in lung and colon cancer cells. However, the role of LINC-PINT in thyroid cancer is unclear. In the present work, we explored the expression of LINC-PINT in 60 paired thyroid cancer and adjacent normal tissues. The clinical significance and biological function of LINC-PINT in thyroid cancer were determined. LINC-PINT expression was downregulated in thyroid cancer relative to adjacent normal tissues (p = 0.0002). Low expression of LINC-PINT was significantly associated with advanced tumor node metastasis (TNM) stage (p = 0.0306) and lymph node metastasis (p = 0.0359). Ectopic expression of LINC-PINT suppressed the proliferation, invasion, and tumorigenesis of thyroid cancer cells. Mechanistically, LINC-PINT associated with and downregulated microRNA (miR)-767-5p. Moreover, LINC-PINT overexpression relieved miR-767-5p-mediated repression of ten-eleven translocation 2 (TET2). miR-767-5p promoted aggressiveness of thyroid cancer, which was reversed by overexpression of TET2. Coexpression of miR-767-5p or depletion of TET2 rescued the inhibitory effect of LINC-PINT on thyroid cancer cell proliferation and invasion. In addition, there was a negative correlation between miR-767-5p and LINC-PINT in thyroid cancer (r = −0.34772, p = 0.01789). Taken together, LINC-PINT functions as a tumor suppressor in thyroid cancer via the miR-767-5p/TET2 axis, representing a potential therapeutic target for thyroid cancer. Graphical Abstract Long noncoding RNA LINC-PINT functions as a tumor suppressor in thyroid cancer. Its downregulation contributes to thyroid cancer growth and progression. Restoration of LINC-PINT provides a potential therapeutic strategy against thyroid cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|