Analysis of adenomatous polyposis coli gene expression, APC locus-microsatellite instability and APC promoter methylation in the progression of melanocytic tumours
| Autor: | Monika Korabiowska, Anegret Müller, Ulrich Brinck, G. Fischer, Nils Siems, Carlos Cordon-Cardo, Thilo Schlott |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Adenomatous polyposis coli Adenomatous Polyposis Coli Protein Loss of Heterozygosity Pathology and Forensic Medicine Familial adenomatous polyposis Loss of heterozygosity 03 medical and health sciences 0302 clinical medicine Gene expression medicine Humans Promoter Regions Genetic Melanoma neoplasms Aged 030304 developmental biology Aged 80 and over 0303 health sciences biology business.industry Microsatellite instability DNA Neoplasm DNA Methylation Middle Aged Prognosis medicine.disease Immunohistochemistry Survival Analysis Lymphatic system Lymphatic Metastasis 030220 oncology & carcinogenesis Multivariate Analysis biology.protein Female business Microsatellite Repeats |
| Zdroj: | Modern Pathology. 17:1539-1544 |
| ISSN: | 0893-3952 |
| Popis: | Adenomatous polyposis coli gene (APC) defects have been demonstrated for the first time in familial adenomatous polyposis. Recent reports indicate that the APC gene is an intermediary between cell adhesion molecules and the cytoskeleton and that it may function as a gatekeeper of colonic epithelial proliferation. The objective of this study was to analyse APC's presence in lentigos, primary melanomas and melanoma metastases. By immunohistochemistry, APC was demonstrated in all lentigos, in 75 out of 88 primary melanomas and in 16 out of 28 melanoma lymphatic metastases. The percentage of immunolabelled tumour cells (APC index) in lentigos ranged between 5 and 69%, in primary melanomas between 0 and 98% and in melanoma metastases between 0 and 52%. Statistically significant differences between lentigos and primary melanomas and between lentigos and metastases in APC expression were found. In a multivariate analysis, APC showed an independent prognostic impact. Analysis of microsatellite instability in the APC locus was performed on 29 melanomas. Microsatellite instability was found in 5/29 melanomas and loss of heterozygosity in 1/29 melanomas. Promoter methylation of APC was found in 6/10 APC-negative primary melanomas and in 9/10 APC-negative melanoma lymphatic metastases investigated. We conclude about important role of APC alterations for melanoma progression. |
| Databáze: | OpenAIRE |
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