Design, construction, and evaluation of a specific chimeric antigen to diagnose chagasic infection
| Autor: | Claudia M. Lagier, María E. Ribone, Ariel Mariano Silber, Iván Sergio Marcipar, Maria Edileuza Felinto de Brito, Sebastian Aguirre |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Chagas disease biology Recombinant Fusion Proteins Trypanosoma cruzi Molecular Sequence Data Toxoplasma gondii Antigens Protozoan biology.organism_classification medicine.disease_cause medicine.disease Fusion protein Virology Sensitivity and Specificity Serology Chimera (genetics) Antigen Streptococcus pyogenes Immunology medicine Animals Humans Chagas Disease Parasitology |
| Zdroj: | Journal of clinical microbiology. 44(10) |
| ISSN: | 0095-1137 |
| Popis: | Chagas' disease is routinely diagnosed by detecting specific antibodies (Abs) using serological methods. The methodology has the drawback of potential cross-reactions with Abs raised during other infectious and autoimmune diseases (AID). Fusion of DNA sequences encoding antigenic proteins is a versatile tool to engineer proteins to be used as sensitizing elements in serological tests. A synthetic gene encoding a chimeric protein containing the C-terminal region of C29 and the N-terminal region of TcP2β was constructed. A 236-serum panel, composed of 104 reactive and 132 nonreactive sera to Chagas' disease, was used to evaluate the performance of the chimera. Among the nonreactive sera, 65 were from patients with AID (systemic lupus erythematosus and rheumatoid arthritis) or patients infected withLeishmania brasiliensis,Brucella abortus,Streptococcus pyogenes, orToxoplasma gondii. The diagnostic performances of the complete TcP2β (TcP2βFL) and its N-terminal region (TcP2βN) were evaluated. TcP2βFLshowed unspecific recognition toward leishmaniasis (40%) and AID Abs (58%), while TcP2βNshowed no unspecific recognition. The diagnostic utility of the chimera was evaluated by analyzing reactivity and comparing the results with those obtained with TcP2βN. The chimera reactivity was higher than that of the peptide fractions (0.874 versus 0.564 optical density,P= 0.0017). The detectability and specificity were both 100% for the whole serum panel tested. We conclude that the obtained chimera shows an improved selectivity and sensitivity compared with other ones previously reported, therefore displaying an optimized performance forTrypanosoma cruziinfection diagnosis. |
| Databáze: | OpenAIRE |
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