Image analyser as a tool for the study ofin vitro glomerular vasoreactivity
| Autor: | B. Lakhdar, L'Azou B, J. Cambar, M. Potier |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Vasodilator Agents Health Toxicology and Mutagenesis Kidney Glomerulus Hemodynamics Renal function Pharmacology urologic and male genital diseases Toxicology Rats Sprague-Dawley Internal medicine Image Processing Computer-Assisted medicine Animals Vasoconstrictor Agents Cells Cultured Cell Size Kidney Microscopy Video Mesangial cell urogenital system Chemistry Angiotensin II Cell Biology Glomerular Mesangium Rats Endocrinology medicine.anatomical_structure Verapamil Cyclosporine medicine.symptom Perfusion Vasoconstriction medicine.drug |
| Zdroj: | Cell Biology and Toxicology. 10:291-295 |
| ISSN: | 1573-6822 0742-2091 |
| DOI: | 10.1007/bf00755772 |
| Popis: | Many drugs used in clinics can dramatically reduce renal hemodynamics. For some years there have been developed in our laboratory two in vitro glomerular models, isolated glomeruli and mesangial cell cultures, to quantitate, by video image analyzer, the direct glomerular effect of vasoreactive agents. The present study shows the vasoconstrictive effects of angiotensin II and cyclosporin in both models and compares their glomerular vasoconstriction with or without vasodilating agents such as verapamil. This drug-induced glomerular vasoreactivity is time- and dose-dependent; moreover, it can be reversible after perfusion in control conditions. The interest of these in vitro glomerular models is validated by fair correlations between in vivo and in vitro data and between the responses of both. These models can be considered as tools for assessing glomerular vasoreactivity of nephrotoxic agents. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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