Effects of transforming growth factor-?1 on the extracellular matrix and cytoskeleton of cultured astrocytes
| Autor: | Michel Pierre, D. Toru-Delbauffe, Jean Michel Gavaret, Denise Baghdassarian |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Phalloidin
Rats Sprague-Dawley Extracellular matrix Cellular and Molecular Neuroscience chemistry.chemical_compound Transforming Growth Factor beta Cerebellum Glial Fibrillary Acidic Protein medicine Animals Cytoskeleton Cells Cultured TGF beta 1 biology Glial fibrillary acidic protein Transforming growth factor beta Molecular biology Actins Extracellular Matrix Fibronectins Rats Cell biology Fibronectin medicine.anatomical_structure Neurology chemistry Astrocytes biology.protein Laminin Cell Division Astrocyte |
| Zdroj: | Glia. 7:193-202 |
| ISSN: | 1098-1136 0894-1491 |
| DOI: | 10.1002/glia.440070302 |
| Popis: | The present study was performed on primary cultures and subcultures of cerebellar astrocytes in order to investigate the effects of transforming growth factor-beta 1 (TGF beta 1) on proliferation, extracellular matrix (ECM) components, and cytoskeletal structures in relation to morphological changes. The expression and cellular distribution of the ECM components laminin and fibronectin and the cytoskeletal proteins glial fibrillary acidic protein (GFAP) and actin were investigated by immunoblotting, immunocytochemistry, and phalloidin staining. The proliferation of primary cultures was strongly inhibited by TGF beta 1. Treated cells became enlarged and spread onto the substratum. TGF beta 1 promoted the appearance of actin stress fibers and increased the cell actin content. It elicited a slight increase in GFAP expression and induced dispersion of thin filaments of GFAP. TGF beta 1 also stimulated the production of laminin and fibronectin and their incorporation into the ECM of primary cultures grown in medium with or without serum. Astrocytes grown in serum-containing medium for 1 day after subculturing responded strongly to TGF beta 1. Changes promoted by TGF beta 1 in cell shape, cytoskeleton, and ECM production of cultured astrocytes may have relevance for understanding the mechanisms of action of TGF beta 1 during brain development. |
| Databáze: | OpenAIRE |
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