Simultaneous quantification of tryptophan metabolites by liquid chromatography tandem mass spectrometry during early human pregnancy
| Autor: | Sofie K M, van Zundert, Pieter H, Griffioen, Lenie, van Rossem, Sten P, Willemsen, Yolanda B, de Rijke, Ron H N, van Schaik, Régine P M, Steegers-Theunissen, Mina, Mirzaian |
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| Přispěvatelé: | Obstetrics & Gynecology, Clinical Chemistry, Epidemiology |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Clinical Chemistry and Laboratory Medicine, 61(3), 442-451. De Gruyter |
| ISSN: | 1437-4331 1434-6621 2022-0790 |
| DOI: | 10.1515/cclm-2022-0790 |
| Popis: | Objectives In this study we describe the development and validation of a liquid chromatography mass spectrometry method (LC-MS/MS) to quantify five tryptophan (TRP) metabolites within the kynurenine– and serotonin pathway and apply the method to serum samples of women in the first trimester of pregnancy. A secondary aim was to investigate the correlation between body mass index (BMI) and the five analytes. Methods A LC-MS/MS was developed for the analysis of TRP, kynurenine (KYN), 5-hydroxytryptophan (5-HTP), hydroxytryptamine (5-HT), and 5-hydroxyindole acetic acid (5-HIAA). Serum samples (n=374) were analyzed of pregnant women (median gestational age: 8 ± 2 weeks) participating in a subcohort of the Rotterdam Periconceptional Cohort (Predict study). Results The LC-MS/MS method provided satisfactory separation of the five analytes (7 min run). For all analytes R2 was >0.995. Within- and between-run accuracies were 72–97% and 79–104%, and the precisions were all Conclusions The LC-MS/MS method is able to accurately quantify kynurenine– and serotonin pathway metabolites in pregnant women, providing an opportunity to investigate the role of the TRP metabolism in the (patho)physiology of pregnancy. |
| Databáze: | OpenAIRE |
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