Glucocorticoid-Induced Apoptosis in CNS Microvascular Pericytes
| Autor: | Andre Katychev, Xueqain Wang, Paula Dore-Duffy, Alexandra Duffy |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Programmed cell death Cell Survival Angiogenesis Neovascularization Physiologic Apoptosis DNA Fragmentation Biology Dexamethasone Rats Sprague-Dawley Receptors Glucocorticoid Glucocorticoid receptor Developmental Neuroscience Annexin Internal medicine medicine Animals Annexin A5 Receptor Glucocorticoids Cells Cultured Dose-Response Relationship Drug Microcirculation Cell Membrane Cytochromes c Cell Differentiation Mitochondria Rats Cell biology Mifepristone Protein Transport Endocrinology medicine.anatomical_structure Neurology Blood-Brain Barrier Female Pericyte Pericytes Glucocorticoid medicine.drug |
| Zdroj: | Developmental Neuroscience. 25:436-446 |
| ISSN: | 1421-9859 0378-5866 |
| DOI: | 10.1159/000075669 |
| Popis: | Pericyte loss or migration from its vascular location may be an important step in microvascular remodeling. Decreased pericyte to endothelial ratios are characteristics of newly formed vessels as well as microvessels undergoing regression, and may be due to selective degeneration via necrotic cell death or via programmed cell death. In this study, we have examined glucocorticoid-induced apoptosis in primary rat CNS pericytes. Characterization of apoptosis was determined using five independent criteria: (1) the translocation of receptors for annexin V from the inner to the outer surface of the plasma membrane, (2) the translocation of cytochrome C from the mitochondria to the cytosol, (3) the induction of DNA fragmentation, (4) the induction of classic changes in cell morphology, and (5) the appearance of TUNEL-positive cells. Incubation of CNS pericytes with dexamethasone induced the appearance of apoptotic cells in a time- and dose-dependent manner. Pericytes express immunologically detectable glucocorticoid receptors, and addition of the glucocorticoid receptor antagonist mifepristone inhibited dexamethasone-induced pericyte apoptosis. That pericytes undergo apoptosis in response to dexamethasone suggests that the regulatory function of this steroid may be important in vascular development and that pericyte apoptotic cell death may accompany vascular regression. Deregulation of pericyte involvement in vascular homeostasis and hemostasis may result in clinical disease. |
| Databáze: | OpenAIRE |
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