A chemiluminescence assay to detect antibodies to brain surface antigens in human sera
| Autor: | Claus Meier, E. Dayrell-Hart, Andreas Zurbriggen, Marc Vandevelde, Martin Brügger |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Immunology
Antibodies law.invention Serology Mice Dogs Antigen law medicine Immunology and Allergy Macrophage Animals Humans Cells Cultured Chemiluminescence Autoimmune disease biology Autoantibody Brain medicine.disease Virology Molecular biology Neurology Cell culture Antigens Surface Luminescent Measurements biology.protein Neurology (clinical) Antibody Nervous System Diseases |
| Zdroj: | Journal of neuroimmunology. 49(1-2) |
| ISSN: | 0165-5728 |
| Popis: | A chemiluminescence assay was developed based on the interaction between antibodies binding to the surface of living brain cells in culture and macrophages. Such interaction leads to production of reactive oxygen radicals which can be measured by a chemiluminescence assay. This assay was used to detect anti brain antibodies in serum samples from humans with various neurological diseases. Such antibody activity was found in a high proportion of these patients. Subsequent experiments with purified IgG fractions and corresponding F(ab′) 2 fragments showed that the observed reactions were highly specific. It was concluded that the chemiluminescence assay is a sensitive and useful technique to detect autoantibodies in neurological diseases. |
| Databáze: | OpenAIRE |
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