High-performance liquid chromatographic separation of kyotorphin, a basic Tyr-Arg dipeptide, in rat brain tissue and quantification using fluorimetric detection
| Autor: | Masaaki Kai, Yosuke Ohkura, Aiko Nakashima |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Brain Chemistry
Cerebral Cortex Male Dipeptide Chromatography Hypothalamus General Chemistry Fluorescence High-performance liquid chromatography Fluorescence spectroscopy Kyotorphin Corpus Striatum Rats Rats Sprague-Dawley chemistry.chemical_compound Spectrometry Fluorescence chemistry Moiety Animals Endorphins Derivatization Quantitative analysis (chemistry) Chromatography High Pressure Liquid Fluorescent Dyes |
| Zdroj: | Journal of chromatography. B, Biomedical sciences and applications. 688(2) |
| ISSN: | 1387-2273 |
| Popis: | Two HPLC procedures based on sample derivatization at the N-terminal Tyr moiety with agents yielding fluorescent derivatives were applied to the selective and sensitive detection as well as quantification of the basic kyotorphin, Tyr-Arg dipeptide, in rat brain tissue. The first one is a post-column fluorescence derivatization method, whereby the peptides extracted from the brain tissue are separated on an octadecylsilyl-silica gel column, followed by on-line fluorescence derivatization for detection. The other one is a pre-column derivatization method, where the extracted peptides are first reacted with fluorogenic agents at the N-terminal Tyr moiety to their corresponding fluorescent derivatives, subsequently separated on an octadecyl-poly(vinyl alcohol) copolymer gel column, and signal responses are measured fluorimetrically. Both methods permitted the quantification of the synthetic kyotorphin added to the rat brain tissues. The concentration range of kyotorphin-like biogenic peptide was 60-100 pmol/g in the cortex, striatum and hypothalamus tissues. |
| Databáze: | OpenAIRE |
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