Quantitative Determination of Nitrofurazone Metabolites in Animal-Derived Foods Based on a Background Fluorescence Quenching Immunochromatographic Assay
| Autor: | Yong Zhou, Yonghua Qi, Jia Wang, Xiaoqi Tao, Xuannian Wang, Licai Ma, Yuping Wu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Health (social science)
Metabolite 02 engineering and technology Plant Science TP1-1185 01 natural sciences Health Professions (miscellaneous) Microbiology Article chemistry.chemical_compound animal-derived foods semicarbazide (SEM) medicine Detection limit Nitrofurazone Quenching (fluorescence) Chromatography medicine.diagnostic_test Chemistry Chemical technology 010401 analytical chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Standard curve Linear range Immunoassay background fluorescence-quenching immunochromatographic assay (bFQICA) quantitative determination 0210 nano-technology Biosensor Food Science |
| Zdroj: | Foods, Vol 10, Iss 1668, p 1668 (2021) Foods Volume 10 Issue 7 |
| ISSN: | 2304-8158 |
| Popis: | Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 μg kg−1 for SEM, respectively, with the linear range of 0.08–0.41 μg L−1, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation < 15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS). |
| Databáze: | OpenAIRE |
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