Novel neutralising antibodies targeting the N-terminal helical region of the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV)
| Autor: | Joachim Denner, Alexander Waechter |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
animal structures
Swine Protein subunit Xenotransplantation medicine.medical_treatment Molecular Sequence Data Immunology medicine.disease_cause Epitope law.invention Viral Envelope Proteins Antigen Neutralization Tests law medicine Animals Amino Acid Sequence Swine Diseases Mutation biology Chemistry Goats Endogenous Retroviruses Viral Vaccines Antibodies Neutralizing Virology Molecular biology Recombinant Proteins Transmembrane protein Mutagenesis Site-Directed biology.protein Recombinant DNA Immunization Rabbits Antibody Epitope Mapping Retroviridae Infections |
| Zdroj: | Immunologic Research. 58:9-19 |
| ISSN: | 1559-0755 0257-277X |
| DOI: | 10.1007/s12026-013-8430-y |
| Popis: | Previously, immunising different species with the transmembrane envelope protein p15E of the porcine endogenous retrovirus (PERV), neutralising antibodies were induced which recognised epitopes in the fusion peptide proximal region (FPPR) and in the membrane-proximal external region (MPER). Only the MPER-specific antibodies were shown to neutralise and these antibodies targeted epitopes in the MPER similarly localised as the epitopes recognised by antibodies broadly neutralising HIV-1 such as 2F5 and 4E10. To study whether neutralising antibodies could be induced immunising with subunits of p15E, recombinant proteins corresponding to the N-terminal, the C-terminal helical region (NHR, CHR) and a p15E with a mutation in the Cys-Cys loop were produced. Whereas none of these antigens induced MPER-specific neutralising antibodies, the animals immunised with the FPPR/NHR subunit and the mutated p15E produced neutralising antibodies binding to the NHR. Therefore, for the first time, antibodies specific for the NHR and neutralising PERV were described. |
| Databáze: | OpenAIRE |
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