| Autor: |
Geng-Xian Shi, F.T. Zhao, Shuzhen Zhang, Li-ping Zhu, Feng‐Rong Ma, Xun Wang, Yi Wang, Guoyan Li, Bo Li |
| Rok vydání: |
1999 |
| Předmět: |
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| Zdroj: |
Science in China. Series C, Life sciences. 43(4) |
| ISSN: |
1006-9305 |
| Popis: |
A 1 846 bp cDNA is isolated from a human tonsil cell lambda gt 11 cDNA library (ATCC No. 37546) with mAb 5D4 reactive strongly with human B cell line 3D5, but weakly with human B cell line Daudi and human T cell line Jurkat as a probe. RT-PCR also shows a strong reaction in 3D5 cell and a weak reaction in Daudi and Jurkat cell for 5D4 mRNA. There is an open reading frame from 88 to 1 209 bp in 5D4 cDNA encoding a 374 AA protein. Both the Northern blot analysis and the two consecutive stop codens before start coden demonstrate that the cDNA is a full-length cDNA. Secondary structure prediction suggests that there are a region from 295 to 334 AA in the protein with strong hydrophobicity and a transmembrane helix region with high score from 313 to 334 AA with an orientation from the inside to the outside of the cell. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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