LC–NMR and LC–MS analysis of 2,3,10,11-oxygenated protoberberine metabolites in Corydalis cell cultures
| Autor: | Wolfgang Wiegrebe, Masataka Moriyasu, Kinuko Iwasa, Makiko Sugiura, Dong-Ung Lee, Ayako Kuribayashi |
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| Rok vydání: | 2003 |
| Předmět: |
Berberine Alkaloids
Plant Science Horticulture Biochemistry Mass Spectrometry Biotransformation Liquid chromatography–mass spectrometry heterocyclic compounds Secondary metabolism Nuclear Magnetic Resonance Biomolecular Molecular Biology Cells Cultured Pseudoprotopine Chromatography Molecular Structure biology Chemistry General Medicine Corydalis Metabolism biology.organism_classification Metabolic pathway Cell culture Oxidation-Reduction Chromatography Liquid |
| Zdroj: | Phytochemistry. 64:1229-1238 |
| ISSN: | 0031-9422 |
| Popis: | The metabolism of 2,3,10,11-oxygenated protoberberine alkaloids was studied in cell cultures of Corydalis species. Without prior isolation, the structures of the metabolites were determined by LC-MS and LC-NMR analyses. Tetrahydropseudocoptisine alpha-N-metho salt, pseudoprotopine, and pseudomuramine were identified for the first time, and preliminary evidence for metabolic pathways to the formation of these alkaloids were obtained. |
| Databáze: | OpenAIRE |
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