Aldo-keto reductase-1 (AKR1) protect cellular enzymes from salt stress by detoxifying reactive cytotoxic compounds

Autor: Jayant K. Solanki, Sukanta K. Sarangi, V. Amarnatha Reddy, Ramu S. Vemanna, Makarla Udayakumar, K. C. Babitha
Rok vydání: 2017
Předmět:
Chlorophyll
0106 biological sciences
0301 basic medicine
Salinity
Antioxidant
Proline
Physiology
medicine.medical_treatment
Protein Carbonylation
Glutathione reductase
Aldo-Keto Reductases
Plant Science
01 natural sciences
Antioxidants
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
Ascorbate Peroxidases
Aldehyde Reductase
Osmotic Pressure
Stress
Physiological
Tobacco
Genetics
medicine
Biomass
Photosynthesis
Aldo-keto reductase
Ornithine-Oxo-Acid Transaminase
biology
Superoxide Dismutase
Methylglyoxal
Salt-Tolerant Plants
Hydrogen Peroxide
Glutathione
Aldehyde Dehydrogenase
Plants
Genetically Modified
Pyruvaldehyde
Glutathione Reductase
030104 developmental biology
chemistry
Biochemistry
biology.protein
Oxidoreductases
010606 plant biology & botany
Peroxidase
Zdroj: Plant Physiology and Biochemistry. 113:177-186
ISSN: 0981-9428
DOI: 10.1016/j.plaphy.2017.02.012
Popis: Cytotoxic compounds like reactive carbonyl compounds such as methylglyoxal (MG), melandialdehyde (MDA), besides the ROS accumulate significantly at higher levels under salinity stress conditions and affect lipids and proteins that inhibit plant growth and productivity. The detoxification of these cytotoxic compounds by overexpression of NADPH-dependent Aldo-ketoreductase (AKR1) enzyme enhances the salinity stress tolerance in tobacco. The PsAKR1 overexpression plants showed higher survival and chlorophyll content and reduced MDA, H2O2, and MG levels under NaCl stress. The transgenic plants showed reduced levels of Na+ levels in both root and shoot due to reduced reactive carbonyl compounds (RCCs) and showed enhanced membrane stability resulted in higher root growth and biomass. The increased levels of antioxidant glutathione and enhanced activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) suggest AKR1 could protect these enzymes from the RCC induced protein carbonylation by detoxification process. The transgenics also showed higher activity of delta 1-pyrroline-5- carboxylate synthase (P5CS) enzyme resulted in increasedproline levels to maintain osmotic homeostasis. The results demonstrates that the AKR1 protects proteins or enzymes that are involved in scavenging of cytotoxic compounds by detoxifying RCCs generated under salinity stress.
Databáze: OpenAIRE
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