Optical Characterization of Parathyroid Tissues
| Autor: | Abraao A. Honorato-Sobrinho, Amando Siuiti Ito, Luciano Bachmann, Luiz Carlos Conti de Freitas, Ricardo Iwakura, Mariana P. Brandao, Kaique Haleplian |
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| Rok vydání: | 2015 |
| Předmět: |
Pathology
medicine.medical_specialty medicine.diagnostic_test Chemistry 0206 medical engineering Thyroid Analytical chemistry Medical evaluation 02 engineering and technology medicine.disease 020601 biomedical engineering 01 natural sciences Fluorescence Fluorescence spectroscopy 010309 optics medicine.anatomical_structure 0103 physical sciences Biopsy medicine Biochemical composition Carcinoma Lymph Instrumentation Spectroscopy GLANDULAS PARATIREOIDES |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1943-3530 |
| Popis: | The parathyroid glands are small and often similar to lymph nodes, fat, and thyroid tissue. These glands are difficult to identify during surgery and a biopsy of the parathyroid for identification can lead to damage of the gland. The use of static and time-resolved fluorescence techniques to detect biochemical composition and tissue structure alterations could help to develop a portable, minimally invasive, and nondestructive method to assist medical evaluation of parathyroid tissues. In this study, we investigated 10 human parathyroid samples using absorbance, fluorescence, excitation, and time-resolved fluorescence measurements. Moreover, we compared the results of time-resolved fluorescence measurements with 59 samples of thyroid tissues. The fluorescence lifetimes with emission at 340 nm were 1.09 ± 0.10 and 4.46 ± 0.06 ns for healthy tissue, 1.01 ± 0.25 and 4.39 ± 0.36 ns for benign lesions, and 0.67 ± 0.36 and 3.92 ± 0.72 ns for malignant lesions. The lifetimes for benign and malignant lesions were significantly different, as attested by the analysis of variance with confidence levels higher than 87%. For each class of samples (healthy, benign, and malignant) we perceived statistical differences between the thyroid and parathyroid tissue, independently. After further investigations, fluorescence methods could become a tool to identify normal and pathological parathyroid tissues and distinguish thyroid from parathyroid tissues. |
| Databáze: | OpenAIRE |
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