Measurements of translation initiation from all 64 codons in E. coli
| Autor: | Jeff E. Glasgow, Lukmaan A. Bawazer, Matthew S. Munson, Drew Endy, Marc L. Salit, Paul R. Jaschke, Ariel Hecht, Jennifer R. Cochran |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Genetics Green Fluorescent Proteins NAR Breakthrough Article Codon Initiator Translation (biology) Biology medicine.disease_cause 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Eukaryotic translation Plasmid chemistry Start codon Escherichia coli medicine Protein biosynthesis Codon Luciferases Peptide Chain Initiation Translational DNA Plasmids |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkx070 |
| Popis: | Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the ‘start codons’ for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 remaining codons. Here, we quantified translation initiation of green fluorescent protein and nanoluciferase in E. coli from all 64 triplet codons and across a range of DNA copy number. We detected initiation of protein synthesis above measurement background for 47 codons. Translation from non-canonical start codons ranged from 0.007 to 3% relative to translation from AUG. Translation from 17 non-AUG codons exceeded the highest reported rates of non-cognate codon recognition. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems. |
| Databáze: | OpenAIRE |
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