Anti-RNA polymerase III antibodies in patients with systemic sclerosis detected by indirect immunofluorescence and ELISA
| Autor: | Christopher C. Bunn, R. W. Burlingame, Jennifer C Parker, T. T. Webb |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Anti-ribonucleic acid polymerase antibody
Anti-nuclear antibody Enzyme-Linked Immunosorbent Assay Sensitivity and Specificity Stain Basic Science Rheumatology Immunopathology Humans Medicine Pharmacology (medical) Fluorescent Antibody Technique Indirect skin and connective tissue diseases Autoantibodies Scleroderma Systemic Indirect immunofluorescence biology business.industry Autoantibody RNA Polymerase III food and beverages IIf biochemical phenomena metabolism and nutrition medicine.disease Connective tissue disease Molecular biology Staining carbohydrates (lipids) Antibodies Antinuclear Immunology biology.protein Systemic sclerosis Antibody business |
| Zdroj: | Rheumatology (Oxford, England) |
| ISSN: | 1462-0332 1462-0324 |
| DOI: | 10.1093/rheumatology/ken201 |
| Popis: | Objectives. To evaluate the analytical performance of an ELISA for the detection of anti-RNA polymerase III antibody (ARA) and to assess IIF as a method for identifying this antibody. Methods. A commercially available ELISA was used to assess the presence of ARA in sera from 1018 SSc patients. The sera had been divided into sub-populations based on the presence of specific autoantibodies, ANA pattern or the absence of both. Patients with ARA (n = 209) had been identified by characteristic ANA pattern by IIF on HEp-2 cell substrate [and additionally by radio-immunoprecipitation (IP) in 157/209 cases]. The remaining 809 SSc patients acted as a control group. Results. Of 157 patients in whom ARA had been confirmed by IP, 150 were positive by ELISA providing a sensitivity of 96%. In the group where ARA had only been assessed by IIF, 100% (52/52) were ELISA positive. The ANA patterns indicating the presence of ARA were a fine-speckled nucleoplasmic stain with additional occasional bright dots, with or without concurrent punctate nucleolar staining. In the SSc control group, the ELISA attained a specificity of 98%, ARA being detected in 17/809 patients. Conclusions. We report the outcome of a study on a large population of SSc patients that shows the ARA ELISA to be of high analytical sensitivity and specificity. We confirm that there is minimal overlap between ARA and other SSc-specific autoantibodies. Additionally, it is demonstrated that the presence of ARA correlates with identifiable patterns by IIF on HEp-2 cell substrate. |
| Databáze: | OpenAIRE |
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