The Novel Protein Complex with SMARCAD1/KIAA1122 Binds to the Vicinity of TSS
| Autor: | Toshihide Yanagawa, Takahiro Nagase, Noriko Okazaki, Reiko Ohara, Shun Ikeda, Hisashi Koga, Kiyo Shimada, Osamu Ohara |
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| Rok vydání: | 2008 |
| Předmět: |
Immunoprecipitation
Molecular Sequence Data Biology Chromatin remodeling Mice Structural Biology Animals Humans Amino Acid Sequence Molecular Biology Phylogeny ChIA-PET Oligonucleotide Array Sequence Analysis Gene Expression Profiling DNA Helicases Nuclear Proteins Neoplasms Germ Cell and Embryonal ChIP-on-chip Molecular biology Leukemia Lymphoid ChIP-sequencing Chromatin Cell biology Gene Expression Regulation Multiprotein Complexes Transcription Initiation Site RIP-Chip Chromatin immunoprecipitation Protein Binding |
| Zdroj: | Journal of Molecular Biology. 382:257-265 |
| ISSN: | 0022-2836 |
| DOI: | 10.1016/j.jmb.2008.07.031 |
| Popis: | The SMARCAD1/KIAA1122 protein is structurally classified into the SWI2/SNF2 superfamily of DNA-dependent ATPases that are catalytic subunits of chromatin-remodeling complexes. Although the importance of other members of the SWR1-like subfamily in chromatin remodeling (EP400, INOC1, and SRCAP) has already been elucidated, the biological function of SMARCAD1/KIAA1122 in transcriptional regulation remains to be clarified. To gain insight into the role of this protein, we generated a specific antibody against SMARCAD1/KIAA1122 and used it for chromatin and protein immunoprecipitation assays. We employed high-resolution genome tiling microarrays in chromatin immunoprecipitation and found the binding sites of SMARCAD1/KIAA1122 in the vicinity of the transcriptional start site of 69 candidate target genes. In the protein immunoprecipitation assay, we found that endogenous SMARCAD1/KIAA1122 binds with TRIM28, a recently highlighted transcriptional regulator in the cancer field. From these findings, we propose a novel model for gene regulation via the SMARCAD1/KIAA1122 protein complex. |
| Databáze: | OpenAIRE |
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