Monoclonal Anti-Lipid A IgM Antibodies HA-1A and E-5 Recognize Distinct Epitopes on Lipopolysaccharide and Lipid A
| Autor: | David C. Morrison, P. E. Daddona, Mei-Guey Lei, W. C. Bogard, Y. Fujihara |
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| Rok vydání: | 1993 |
| Předmět: |
Lipopolysaccharides
Idiotype Lipopolysaccharide medicine.drug_class Enzyme-Linked Immunosorbent Assay Monoclonal antibody Binding Competitive Epitope Lipid A Epitopes Mice chemistry.chemical_compound Immunoglobulin Idiotypes Antigen Antibody Specificity medicine Animals Humans Immunology and Allergy biology Antibodies Monoclonal Molecular biology Infectious Diseases Immunoglobulin M chemistry biology.protein Antibody |
| Zdroj: | Journal of Infectious Diseases. 168:1429-1435 |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/168.6.1429 |
| Popis: | Specific binding of two monoclonal IgM antibodies previously investigated as therapeutic agents for treating gram-negative septic shock, HA-1A and E5, was assessed with respect to lipid A and lipopolysaccharide (LPS). Both antibodies bound to lipid A; however, binding of HA-1A was significantly greater than that of E5 to LPS derived from rough strains of bacteria. Reciprocal competitive inhibition experiments supported the concept that HA-1A and E5 bind to distinct epitopes on lipid A. Further, competitive inhibition studies using a monoclonal anti-idiotype antibody with specificity for the variable region of HA-1A suggested that HA-1A and E5 do not share a common idiotype. Finally, studies using double-stranded DNA as antigen indicated that E5 but not HA-1A will bind to DNA. Collectively, these data indicate that HA-1A and E5 are different lipid A-specific antibodies that bind to distinct epitopes on lipid A. |
| Databáze: | OpenAIRE |
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