Polymorphisms of sperm protamine genes and CMA3 staining in infertile men with varicocele
| Autor: | Seyed Morteza Seifati, Nasim Tabibnejad, Maryam Nayeri, Mohammad Mehdi Heidari, Ali Reza Talebi |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Infertility Heterozygote Urology Varicocele Male infertility Andrology 03 medical and health sciences 0302 clinical medicine medicine Humans Protamines Cell Shape Gene Infertility Male Fluorescent Dyes Polymorphism Genetic 030219 obstetrics & reproductive medicine Sperm Count Staining and Labeling biology business.industry Homozygote Case-control study Chromomycin A3 medicine.disease Spermatozoa Protamine Sperm Staining 030104 developmental biology Reproductive Medicine Case-Control Studies Sperm Motility biology.protein business |
| Zdroj: | Revista Internacional de Andrología. 18:7-13 |
| ISSN: | 1698-031X |
| Popis: | Objectives The aim of this study was to evaluate polymorphisms of sperm protamine genes and their effects on the result of CMA3 staining in varicocele men. Material and methods In a case control study, 128 patients with male infertility due to varicocele and 128 controls were recruited. Polymorphisms of PRM1 and PRM2 genes in extracted DNA samples were assessed by PCR-SSCP and sequencing. Protamine deficiency was also indirectly estimated by CMA3 staining. Result Nine different variants including six variants in PRM1 gene and three variants in PRM2 gene were found among varicocele patients. The results showed that sperm count, motility and morphology were significantly different between control group without gene variations and varicocele group who had several variations in their protamine genes (P Conclusion Therefore, PRM1 and PRM2 variations in varicocele patients are associated with the production of spermatozoa with more protamine deficiency and this is one of the possible causes of infertility due to varicocele. |
| Databáze: | OpenAIRE |
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