Raman microspectrometry of laser-reshaped rabbit auricular cartilage: preliminary study on laser-induced cartilage mineralization
| Autor: | Gérard Leroy, Laurence Fleurisse, Serge Mordon, Collette Creusy, Michal Heger |
|---|---|
| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Biomedical Engineering
chemistry.chemical_element Pilot Projects Calcium Spectrum Analysis Raman Apatite Calcium in biology Biomaterials Extracellular matrix Extracellular medicine Cartilaginous Tissue Animals Minerals biology Chemistry Cartilage Anatomy Plastic Surgery Procedures Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Treatment Outcome medicine.anatomical_structure Proteoglycan visual_art biology.protein visual_art.visual_art_medium Biophysics Female Laser Therapy Rabbits Ear Cartilage |
| Zdroj: | Journal of biomedical optics, 11(2). SPIE |
| ISSN: | 1083-3668 |
| DOI: | 10.1117/1.2187420 |
| Popis: | Laser-assisted cartilage reshaping (LACR) is a relatively novel technique designed to noninvasively and permanently restructure cartilaginous tissue. It is believed that heat-induced stress relaxation, in which a temperature-mediated disruption of H2O binding is associated with conformational alterations in the proteoglycan and collagen-rich matrix, constitutes the underlying mechanism of LACR. Several reports have suggested that laser-mediated cartilage mineralization may contribute to the permanent shape change of laser-reshaped cartilage. In an effort to validate these results in the context of Er:glass LACR, we performed a preliminary Raman microspectrometric study to characterize the crystal deposits in laser-irradiated chondrocytes and extracellular matrix. For the first time, we identified intracellular calcium sulfate deposits and extracellular calcium phosphate (apatite) crystals in laser-reshaped rabbit auricular cartilage. Calcium carbonate deposits are localized in both irradiated and nonirradiated samples, suggesting that this mineral plays no role in conformational retention. In our discussion, we elaborate on the possible molecular and cellular mechanisms responsible for intra- and extracellular crystallization, and propose a novel hypothesis on the formation of apatite, inasmuch as the biological function of this mineral (providing structure and rigidity in bones and dental enamel) may be extrapolated to the permanent shape change of laser-irradiated cartilage. |
| Databáze: | OpenAIRE |
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