SmLEA2, a gene for late embryogenesis abundant protein isolated from Salvia miltiorrhiza, confers tolerance to drought and salt stress in Escherichia coli and S. miltiorrhiza
| Autor: | Xiaorong Guo, Xinbing Yang, Yucui Wu, Xiaoyan Cao, Huaiqin Wang |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Transgene Salvia miltiorrhiza Plant Science Genetically modified crops Biology Sodium Chloride medicine.disease_cause Genes Plant 01 natural sciences Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Stress Physiological Botany medicine Escherichia coli Phylogeny Plant Proteins Abiotic component Microbial Viability Abiotic stress fungi Sodium food and beverages Plant Transpiration Cell Biology General Medicine Plants Genetically Modified Adaptation Physiological Droughts 030104 developmental biology Phenotype chemistry Potassium Electrophoresis Polyacrylamide Gel 010606 plant biology & botany |
| Zdroj: | Protoplasma. 254(2) |
| ISSN: | 1615-6102 |
| Popis: | Abiotic stresses, such as drought and high salinity, are major factors that limit plant growth and productivity. Late embryogenesis abundant (LEA) proteins are members of a diverse, multigene family closely associated with tolerance to abiotic stresses in numerous organisms. We examined the function of SmLEA2, previously isolated from Salvia miltiorrhiza, in defense responses to drought and high salinity. Phylogenetic analysis indicated that SmLEA2 belongs to the LEA_2 subfamily. Its overexpression in Escherichia coli improved growth performance when compared with the control under salt and drought stresses. We further characterized its roles in S. miltiorrhiza through overexpression and RNAi-mediated silencing. In response to drought and salinity treatments, transgenic plants overexpressing SmLEA2 exhibited significantly increased superoxide dismutase activity, reduced levels of lipid peroxidation, and more vigorous growth than empty-vector control plants did. However, transgenic lines in which expression was suppressed showed the opposite results. Our data demonstrate that SmLEA2 plays an important role in the abiotic stress response and its overexpression in transgenic S. miltiorrhiza improves tolerance to excess salt and drought conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink