Evaluation of a Chlamydophila psittaci infection diagnostic platform for zoonotic risk assessment
| Autor: | Kristel Verminnen, Daisy Vanrompay, Birgitta Duim, David De Keukeleire, Yvonne Pannekoek, Barbara Duquenne, Lutgart Braeckman |
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| Přispěvatelé: | Medical Microbiology and Infection Prevention, AII - Amsterdam institute for Infection and Immunity |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Male Turkeys Genotype Fluorescent Antibody Technique Psittacosis Polymerase Chain Reaction Risk Assessment Serology Belgium Zoonoses medicine Animals Humans Chlamydiaceae Poultry Diseases Chlamydia psittaci Bird Diseases Chlamydophila biology Transmission (medicine) Bacteriology Sequence Analysis DNA biology.organism_classification medicine.disease bacterial infections and mycoses Virology Bacterial Typing Techniques Chlamydophila psittaci Chlamydiales Immunology Female Bacterial Outer Membrane Proteins |
| Zdroj: | Journal of clinical microbiology, 46(1), 281-285. American Society for Microbiology |
| ISSN: | 0095-1137 |
| Popis: | Reports on zoonotic transmission of Chlamydophila psittaci originating from poultry are incidentally published. During recent studies in European turkeys we isolated C. psittaci genotypes A, B, D, E, F, and E/B, all considered potentially dangerous for humans. This encouraged us to analyze the zoonotic risk on a Belgian turkey farm, from production onset until slaughter, using a Chlamydophila psittaci diagnostic platform. Twenty individually marked hens, as well as the farmer and two scientists, were monitored medically. Bioaerosol monitoring, serology, isolation, and nested PCR demonstrated chlamydiosis on the farm leading to symptomatic psittacosis in all 3 persons involved. ompA sequencing confirmed the zoonotic transmission of C. psittaci genotype A. Strangely, two different antibody microimmunofluorescence (MIF) tests remained negative in all infected persons. The results demonstrate the value of the currently used diagnostic platform in demonstrating C. psittaci infections in both birds and humans but raise questions regarding use of the MIF test for diagnosing human psittacosis. In addition, our results suggest the underestimation of psittacosis in the poultry industry, stressing the need for a veterinary vaccine and recommendations for zoonotic risk reduction strategies. |
| Databáze: | OpenAIRE |
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