Analysis of antibiotics in urine and wipe samples from environmental and biological monitoring--comparison of HPLC with UV-, single MS- and tandem MS-detection
| Autor: | Jochen Tuerk, Dennis Dreyer, Thekla K. Kiffmeyer, Marius Reinders, Heinz-Martin Kuss, Klaus Gerhard Schmidt |
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| Rok vydání: | 2004 |
| Předmět: |
Detection limit
Chromatography Chemistry Clinical Biochemistry Reproducibility of Results Cell Biology General Medicine Urine Environmental Exposure Mass spectrometry Tandem mass spectrometry Biochemistry Trimethoprim High-performance liquid chromatography Sensitivity and Specificity Mass Spectrometry Analytical Chemistry Anti-Bacterial Agents Matrix (chemical analysis) Liquid chromatography–mass spectrometry medicine Spectrophotometry Ultraviolet Chromatography High Pressure Liquid medicine.drug |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 831(1-2) |
| ISSN: | 1570-0232 |
| Popis: | Results of the simultaneous determination of the structurally different antibiotics cefazoline, cefotiame, cefuroxime, chloramphenicol, ciprofloxacin, ofloxacin, sulfamethoxazole and trimethoprim from environmental and biological monitoring using high-performance liquid chromatography with UV, single mass and tandem mass spectrometry were compared. For sample enrichment and clean-up a SPE method using bakerbond C18 cartridges was developed. Mean recovery rates were above 70%. Because of the complex urine matrix, only the wipe samples could be analyzed by UV-detection. However, UV-detection and single MS-detection are useful for control measurements after spillage, e.g. (LOD = 1–2 ng/cm 2 ). Samples from biological monitoring of occupational uptake should be analyzed by LC–MS/MS. The limits of detection (LOD) in urine ranged from 0.4 to 70 μg/L for LC–MS and 0.01 to 0.9 μg/L for LC–MS/MS detection. The limits of detection in wipe samples ranged from 0.003 to 0.13 ng/cm 2 . |
| Databáze: | OpenAIRE |
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