Seasonal variation of pituitary gonadotropin subunit, brain-type aromatase and sex steroid receptor mRNAs, and plasma steroids during gametogenesis in wild sablefish
| Autor: | Penny Swanson, J. Adam Luckenbach, Frederick W. Goetz, Gina M. Ylitalo, Edward S. Hayman, Denis A.M. da Silva, José M. Guzmán |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine endocrine system medicine.medical_specialty Physiology medicine.drug_class Estrogen receptor 030209 endocrinology & metabolism Biochemistry FSHB Vitellogenins 03 medical and health sciences Aromatase Oogenesis 0302 clinical medicine Tandem Mass Spectrometry Internal medicine medicine Animals RNA Messenger Gonadal Steroid Hormones Spermatogenesis Molecular Biology Gametogenesis biology Fishes Brain Androgen biology.organism_classification Spermatozoa 030104 developmental biology Endocrinology Receptors Estrogen Receptors Androgen Sex steroid Pituitary Gland Gonadotropins Pituitary biology.protein Female Steroids Seasons Vitellogenesis Sablefish |
| Zdroj: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. :48-57 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2018.02.010 |
| Popis: | Pituitary-hormone signaling plays critical roles in the onset and progression of gametogenesis in vertebrates. This study characterized expression patterns of pituitary gonadotropin beta-subunits (fshb and lhb), brain-type aromatase (cyp19a1b), androgen (ar1, ar2) and estrogen receptors (esr1, esr2a, esr2b), and changes in plasma steroid levels by liquid chromatography/tandem mass spectrometry in wild sablefish (Anoplopoma fimbria, order Scorpaeniformes) during a complete reproductive cycle. Transcripts for fshb increased during early gametogenesis and peaked in late vitellogenic females and late recrudescent males, while expression of lhb reached maximum levels in periovulatory and spermiating fish. Pituitary levels of cyp19a1b and ar1 were strongly correlated with those of lhb in females and males, increasing during gametogenesis and reaching maximum levels prior to spawning. By contrast, expression of ar2, and the three estrogen receptors differed between female and male sablefish. 17β-estradiol (E2) was the dominant steroid in females during vitellogenesis, while a range of at least 6 steroids (11β-hydroxyandrostenedione, testosterone [T], E2, 11-ketotestosterone [11KT], 11-deoxycortisol, and 17α,20β,21-trihydroxyprogesterone) were detected at similar levels in males during testicular development. Prior to spawning, a marked increase in 4-androstenedione, T, 11KT and E2 was found in both periovulatory females and spermiating males. In conclusion, the concomitant changes in plasma androgen levels and pituitary ar1 expression during gametogenesis suggest a specific role for androgens in pituitary hormone regulation of reproduction in sablefish. Further, our data highlight the importance of E2 during final stages of maturation in this species, which may regulate the transcription of pituitary lhb in a paracrine fashion. |
| Databáze: | OpenAIRE |
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