Decreased sialylation of the acute phase protein α1-acid glycoprotein in feline infectious peritonitis (FIP)
| Autor: | Fabrizio Ceciliani, Saverio Paltrinieri, Claudia Grossi, Alessia Giordano, Vanessa Pocacqua |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Male
Feline coronavirus Glycosylation MAA Maackia amurensis agglutinin Blotting Western Immunology α1-Acid glycoprotein Biology medicine.disease_cause Article HPLC high pressure liquid chromatography Feline Infectious Peritonitis chemistry.chemical_compound Agglutinin Carbohydrate Conformation medicine Animals Coronavirus Feline FIP feline infectious peritonitis Fucosylation chemistry.chemical_classification AAL Aleuria aurantia lectin SleX sialyl Lewis X General Veterinary FCoV feline coronavirus SNAI Sambucus nigra agglutinin fAGP feline α1-acid glycoprotein Lectin HP haptoglobin Orosomucoid Chromatography Ion Exchange Feline infectious peritonitis Sialic acid chemistry Biochemistry AGP α1-acid glycoprotein Cats Sialic Acids biology.protein Female Plant Lectins Glycoprotein |
| Zdroj: | Veterinary Immunology and Immunopathology |
| ISSN: | 0165-2427 |
| DOI: | 10.1016/j.vetimm.2004.02.003 |
| Popis: | Feline infectious peritonitis (FIP) is an immune-mediated disease of domestic and exotic felides infected with feline coronavirus. FIP is characterized by the overexpression of an acute phase protein, the alpha1-acid glycoprotein (AGP). In humans, AGP is a heavily glycosylated protein that undergoes several modifications of its glycan moiety during acute and chronic inflammatory pathologies. We studied the changes in AGP glycosylation in the course of FIP. Specifically, we focussed our attention on the degree of sialylation, fucosylation and branching. This study presents a purification method for feline AGP (fAGP) from serum, using an ion exchange chromatography strategy. The glycosylation pattern was analyzed in detail by means of interaction of purified fAGP with specific lectins. In particular, Sambucus nigra agglutinin I and Maackia amurensis agglutinin lectins were used to detect sialic acid residues, Aleuria aurantia lectin was used to detect L-fucose residues and Concanavalin A was used to evaluate the branching degree. By this method we showed that fAGP did not present any L-fucose residues on its surface, and that its branching degree was very low, both in normal and in pathological conditions. In contrast, during FIP disease, fAGP underwent several modifications in the sialic acid content, including decreased expression of both alpha(2-6)-linked and alpha(2-3)-linked sialic acid (76 and 44%, respectively when compared to non-pathological feline AGP). |
| Databáze: | OpenAIRE |
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