Fibrinogen/thrombin-based collagen fleece (TachoComb(R)) promotes regeneration in pulmonary arterial injury
| Autor: | Morihito Okada, Koji Arihiro, Takuhiro Ikeda, Yoshihiro Miyata, Yasuhiro Tsutani, Keizo Misumi |
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| Rok vydání: | 2011 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Endothelium Blood Loss Surgical Drug Evaluation Preclinical Pulmonary Artery Hemostatics Fibrin Aprotinin Dogs medicine.artery medicine Animals Regeneration Hemostatic function Wound Healing biology Thoracic cavity business.industry Thrombin Fibrinogen General Medicine medicine.disease Hemostasis Surgical Surgery Disease Models Animal Drug Combinations Stenosis medicine.anatomical_structure Thoracotomy Cardiothoracic surgery Hemostasis Pulmonary artery biology.protein Feasibility Studies Female Collagen Cardiology and Cardiovascular Medicine business |
| Zdroj: | European Journal of Cardio-Thoracic Surgery. 41:926-932 |
| ISSN: | 1873-734X 1010-7940 |
| Popis: | OBJECTIVES: To repair unexpected damage of the pulmonary artery (PA) during thoracic surgery, fibrinogen/thrombin-based collagen fleece (TachoComb ® [TC]) can be applied as a haemostatic material. The progression of vessel restoration with TC has not been elucidated. In this study, we investigate details of the healing process with TC after PA injury using a canine model. METHODS: Left thoracotomy was performed on female beagles under general anaesthesia. PA injury was induced and repaired using TC. Repair sites were histologically evaluated 2, 4 and 8 weeks after surgery (n= 3 in each group). RESULTS: Haemostasis of PA injury was achieved promptly after TC application. After surgery, no bleeding was found in the thoracic cavity, and no repair sites revealed stenosis, thrombi or false aneurism formation. Two weeks after surgery, inflammatory cells had infiltrated around the vascular defect, and vascular endothelium had regenerated on the innermost surface of TC applied to the defect. At Week 4, elastic and smooth muscle fibres had begun to extend into the defect between the endothelial layer and collagen fleece. By Week 8, elastic fibres and smooth muscle had completely regenerated in the medial layer. The adventitial layer had also fully regenerated. CONCLUSIONS: Haemostasis of injured PA using TC was safe and reliable. TC provided a mechanical scaffold on which vascular regeneration occurred. Three layers reconstructed in the PA defect were identical to those in normal structures. |
| Databáze: | OpenAIRE |
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